摘要
为了探明丝瓜核心种质资源的亲缘关系,本研究利用17对SRAP标记和1对来源于黄瓜的EST-SSR标记,对42份国内外丝瓜资源材料进行了遗传多样性分析。18个引物组合共扩增出197个位点,其中多态性位点为156个,多态位点比率79.18%。每个引物组合扩增位点数在6~16个之间,平均每个引物组合扩增位点数为10.94;通过NTSYS软件计算得到的样品间SM相似系数介于0.272~0.991,说明丝瓜属的遗传变异范围较大。根据SRAP扩增结果,利用UPGMA法构建树状聚类图,聚类分析将42份样品分为2组,一组包含27个有棱丝瓜品种,另一组包含15个普通丝瓜品种,种群间遗传差异大,同一品种群内遗传差异较小,聚类分析结果与丝瓜的形态分类系统基本相符。同时,对SRAP标记在丝瓜种质资源中的扩增重复性进行了检测,表明SRAP标记具有稳定的重现性。
In order to evaluate the genetic relationship among germplasm of sponge gourd,17 sequence related amplified polymorphism（ SRAP） markers and one EST-SSR marker derived from cucumber were used to evaluate the genetic diversity among 42 sponge gourd accessions. Total 197 bands were amplified using 18 primer pairs,156 bands（ 79. 18 %） of which were polymorphic ones. The number of loci detected by per primer pairs was range from 6 to16,which the average was 10. 94（ with an average of 0. 733）. Genetic similarity coefficients（ SM coefficient） of 42 sponge gourd accessions ranged from 0. 272 to 0. 991. A dendrogram was constructed based on SRAP data using UPGMA cluster method. The 42 sponge gourd accessions were divided into 2 major clusters,one comprising of 27 accessions of Luffa acutangula（ L.） Roxb. and the other of 15 accessions belonging to Luffa cylindrical（ L.） Roem. The two clusters（ inter-species） were very far in genetic relationship,whereas the intra-species accessions had extremely close genetic distance. This result was basically corresponded with the genetic relationships based on morphological traits. Meanwhile,the repeatability of SRAP markers in sponge gourd were detected,indicating that the SRAP markers had stable reproducibility.
出处
《西南农业学报》
CSCD
北大核心
2015年第4期1524-1529,共6页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31401865)
广东省科技项目(2012A020100006
2013A061402002
2013B060400040
2014B020202006
2014B020202007
2014A020209055
2012A020100006)
国家现代农业产业技术体系建设专项(CARS-25-G-36)
广东省农业科学院蔬菜研究所所长基金项目(所-201403)
