摘要
目的观察射干麻黄汤对寒哮患者外周血T细胞亚群及白细胞介素(interleukin,IL)-5、IL-10 mRNA水平的影响。方法将51例轻中度寒哮患者按随机数字表法分为治疗组25例和对照组26例。对照组给沙丁胺醇气雾剂,每次吸入100μg,每天4次;治疗组在对照组的基础上加用射干麻黄汤中药配方颗粒冲服,每天1剂,早晚2次冲服。两组均治疗12周。分别于治疗前和治疗后12周留取血液行流式细胞术检测T细胞亚群,RT-PCR检测IL-5、IL-10 mRNA表达。结果治疗后两组比较:CD3+、CD4+、CD8+无显著差异,CD4/CD8比值(t=2.593,P=0.012)、IL-5(t=2.492,P=0.016)明显下降,IL-10(t=2.659,P=0.011)明显上升。结论射干麻黄汤调节哮喘患者T细胞CD4/CD8比例及IL-5、IL-10表达水平,改变机体细胞免疫功能,控制哮喘症状。
Objective To observe the effects of Shegan Mahuang decoction on peripheral blood T cell subsets and IL-5mRNA, IL-10mRNA expression in asthmatics. Methods 51 cases of mild to moderate asthmatics were randomly divided into treatment group （ 25 patients ） and control group （ 26 patients） . Control group was given salbutamol aerosol, each inhalation 100μg, four times daily;treatment group were given extra dosage of Chinese medicine （ Shegan Mahuang decoction） , twice a day on in the morning and evening. Both groups were treated for 12 weeks. Blood specimens were collected respectively before and after treatment. Then T cell subsets were detected by the line flow cytometry, and IL-5, IL-10mRNA expression tested by RT-PCR. Results Compared with control group, T cell subsets CD3＋, CD4＋, CD8＋ showed no significant difference, but CD4/CD8 ratio （t = 2.593, P = 0.012）, IL-5 （t= 2. 492, P= 0. 016） and IL-10 （t= 2. 659, P= 0. 011） were changed significantly. Conclusion Shegan Mahuang decoction can adjust CD4/CD8 ratio and the IL-5, IL-10 expression, regulate immune function, and control asthma symptoms.
出处
《环球中医药》
CAS
2015年第8期912-915,共4页
Global Traditional Chinese Medicine
基金
武汉市卫生局中医药中西医结合科研课题(武卫[2007]43号)
