摘要
目的:以大脑中动脉闭塞(MCAO)大鼠模型为研究对象,探讨针刺对脑卒中后痉挛大鼠纹状体和脊髓中兴奋性氨基酸和抑制性氨基酸含量的影响。方法:雄性SD大鼠68只。随机分为3组,正常组9只、假手术组9只、造模组50只。其中,造模组采用改良线栓法制备大鼠MCAO模型,运用Zea Longa 5分制评分标准进行神经行为学评分。模型制作成功后,随机分为模型组、针刺组和巴氯芬组。在痉挛未发生的软瘫期(造模后第2天)对以上5组大鼠进行相关干预。干预7d后,检测大鼠纹状体和脊髓中谷氨酸(Glu)、天冬氨酸(Asp)、甘氨酸(Gly)含量的变化。结果:各组大鼠纹状体中兴奋性氨基酸和抑制性氨基酸的变化:与正常组比较,模型组抑制性氨基酸Gly显著下降(P<0.05)。与模型组比较,针刺组兴奋性氨基酸Glu含量显著下降(P<0.01)。各组大鼠脊髓中兴奋性氨基酸和抑制性氨基酸的变化:与正常组比较,模型组兴奋性氨基酸Asp和Glu、抑制性氨基酸Gly均显著下降(P<0.05,P<0.01)。与模型组比较,针刺组、巴氯芬组3种氨基酸含量均无明显差异。结论:针刺王氏夹脊穴可通过降低兴奋性氨基酸谷氨酸的含量,从而发挥抗痉挛效应。
Objective： To explore the influence of acupuncture on the the amino acid content in the striatum and spinal cord in MCAO rat. Methods： Sixty-eight healthy male Sprague-Dawley rats were randomized into 3 groups： normal group （n=9）, fake surgery group （n=9）, and the model group （n=50）. In the model group, rat models of the middle cerebral artery occlusion （MCAO） was established using the intraluminal filament occlusion; Zea Longa score was used to assess rats＇ neurological symptoms and behaviors. Once the rat model of MCAO was established, the rat models were randomized into no intervention group, acupuncture group, and baclofen group. Since two days after stroke is in the flaccidity phase, all rats in these five groups were treated with corresponding procedures. After seven days of treatment, glutamine （Glu）, aspatate （Asp）, glycine （Gly） levels were assessed. Results： The changes of excitatory amino acids and inhibitory amino acids in the striatum： Compared with the normal group, Gly levels in the rat model group was significantly decreased in the striatum （P〈0.05）. Compared with the model group, rats receiveing acupuncture treatment had significantly lower Glu concentrations in the striatum （P〈0.01）. The changes of excitatory amino acids and inhibitory amino acids in the spinal cord： Compared with the normal group, Asp, Glu, Gly levels in the rat model group were significantly decreased （P〈0.05, P〈0.01）. Compared with the model group, Asp, Glu, Gly levels in rats of the acupuncture group and baclofen group don＇t change significantly. Conclusion： Acupuncture at Jiaji point could exert antispasmodic effects by reducing excitability amino acid content.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第9期3105-3107,共3页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金面上项目(No.81072859)
北京市自然科学基金青年项目(No.7144215)
北京市教委科技发展计划面上项目(No.KM201210025022)~~
关键词
脑卒中
痉挛
针刺
王氏夹脊穴
氨基酸
Stroke
Spasticity
Acupuncture
Jiaji points
Amino acids
