摘要
背景:黄韧带是脊柱后部的重要连接结构,一旦出现退变导致其弹性下降,在各种影响因素作用下,可使椎管的有效空间更加减小,导致脊髓、神经根受压,出现神经症状,严重者可致瘫痪、病残。目的:分析过量氟化物导致大鼠腰椎黄韧带退变与骨化的作用机制。方法:选取雄性SD大鼠30只,随机分为两组,观察组饮用含Na F(质量分数为10-4)蒸馏水,对照组给予等量的蒸馏水,分别于3,6个月后检测骨密度,血清、骨组织标本中Ca2+、P3+含量和血清碱性磷酸酶活性;且行大鼠腰段黄韧带标本X射线检查后组织病理观察。结果与结论:观察组3个月后有5只大鼠下门牙开始出现白垩色条纹或斑点,6个月后大鼠均出现氟斑牙,表现为广泛的白垩色,且大鼠中腰椎骨密度显著增加(P<0.05);观察组大鼠在3个月和6个月时其血清Ca2+均显著高于对照组,P3+显著低于对照组,差异均有显著性意义(P<0.05);且血清碱性磷酸酶的活性在3个月和6个月时均显著高于对照组(P<0.05);6个月后X射线检查发现大鼠腰椎骨质密度明显增高;病理观察结果显示观察组大鼠黄韧带中弹力纤维数量进一步减少,胶原纤维大量增生,大量的成纤维细胞与钙盐沉积,且在椎板附着处可见韧带骨化形成。结果提示过量氟化物可造成SD大鼠腰椎黄韧带的退变、骨化,可能在黄韧带的骨化中可能起重要作用。
BACKGROUND： The ligamentum flavum is important to connect the rear of the spine. If the flexibility of the ligamentum flavum is decreased due to degeneration, in the role of various factors, it can reduce the effective space of the spinal canal to induce spinal cord and nerve root compression, thereby resulting in neurological symptoms, even paralysis or residual disease. OBJECTIVE： To study the mechanism of over-dose fluoride on the degeneration and ossification of the ligamentum flavum in rats.METHODS： Thirty male Sprague-Dawley rats were randomly divided into two groups： experimental group was given distilled water containing NaF （mass fraction 104） and control group was given the same amount of distilled water. After 3 and 6 months, the bone mineral density, Ca^2＋ and P^3＋ contents in serum and bone tissue and serum alkaline phosphatase activity were detected, respectively. Meanwhile, the ligamentum flavum specimens were taken for X-ray examination and histopathological observation. RESULTS AND CONCLUSION： In the experimental group, chalk stripes or spots occurred in the lower incisors of five rats at 3 months after treatment and dental fluorosis that was mostly in chalk color was found in all the rats at 6 months after treatment; moreover, the bone density of the lumbar spine significantly increased （P 〈 0.05）. Compared with the control group, the contents of Serum Ca^2＋ were significantly higher, while the contents of P^3＋ were significantly lower in the experimental group at 3 and 6 months after treatment （P 〈 0.05）; the serum alkaline phosphatase activity was also significantly higher in the experimental group than the control group at 3 and 6 months after treatment （P 〈 0.05）. X-ray films, at 6 months after treatment, showed that the bone density of the lumbar vertebra was increased obviously; pathological observation showed that the number of elastic fibers in the ligamentum flavum was reduced further, a large amount of collagen fibers, fibroblasts and calcium deposition were found, and the ossification of the ligamentum flavum was visible at the lamina attachment site. These findings indicate that the over-dose fluorides can lead to the degeneration and ossification of the ligamentum flavum in Sprague-Dawley rats, which may plan an important role in the ossification of the ligamentum flavum.
出处
《中国组织工程研究》
CAS
北大核心
2015年第33期5301-5305,共5页
Chinese Journal of Tissue Engineering Research
