摘要
采用Trizol法、CTAB法、RNA prep Pure Plant Kit方法分别从萼脊兰的根、叶、花葶、花萼、花瓣、唇瓣、柱头中提取总RNA,比较3种总RNA提取方法的优劣,分析所提取萼脊兰不同组织总RNA质量高低。结果显示:CTAB法提取的RNA得率最高,完整性好,凝胶电泳泳道无杂质,条带单一,可以满足荧光定量PCR的试验需要。
The total RNA of sedirea japonica from the root, leaf, pedicel, sepal, petal, column and labellum was extracted by Trizol method, CTAB method and RNA prep Pure Plant Kit, respectively. The and quality of total RNA extracted from different tissues was compared and analyzed. The results showed that the best was CTAB method which had high yield, high integrity, clear and single strip in the gel eleetrophoresis, and could meet the needs of the real time quantitative PCR test.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2015年第4期468-471,共4页
Journal of Henan Agricultural University
基金
郑州市科技计划项目(112PPTGY250-3)
关键词
萼脊兰
不同组织
总RNA提取方法
sedirea japonica
different tissue
total RNA extraction method
