摘要
目的:探讨风轮菜总黄酮的不同极性部位对缺氧/复氧诱导的H9c2心肌细胞损伤的保护作用。方法:建立缺氧/复氧H9c2心肌细胞损伤模型,对风轮菜总黄酮10%甲醇部位(2#)、20%甲醇部位(3#)、30%甲醇部位(5#)进行药物浓度筛选,采用MTT法测定细胞存活率。收集培养细胞及其上清液测定乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化氢酶(CAT)的活性和丙二醛(MDA)的含量。结果:与正常对照组比,模型组H9c2心肌细胞经过缺氧4h复氧24h造模后,细胞活力显著降低。与模型组比较,2#、3#、5#给药组显著增加H9c2心肌细胞活力。与正常对照组比较,模型组的SOD、GSH-Px及CAT的活性均显著降低,而LDH活性、MDA含量均升高,2#、3#、5#给药组可呈浓度依赖性显著增加SOD、GSH-Px及CAT活性,降低LDH活性及MDA含量。结论:2#、3#、5#对缺氧/复氧诱导的H9c2心肌细胞损伤具有保护作用,其与增强细胞清除氧自由基能力、降低脂质过氧化物的产生有关。
Objective：To explore the protective effect of different polar fractions in total flavones of Clinopodium chinenseon on H9c2 myocardial cell damage induced by hypoxia/reoxygenation（H/R）.Methods：The hypoxia/reoxygenation injury cardiomyo-cyte model was established and screened out parts with 1 0%（2#）,20%（3#）,30%（5 #）of carbinol.Cell viability was deter-mined by MTT method.We collected culture cell and its supernatant for detecting LDH,SOD,GSH-Px,CAT activity and MDA content.Results：In H/R group,after hypoxia 4 h and reoxygenation 24 h,the myocardial cell viability decreased significantly compared to control group.Compared with H/R group,2 #、3 #、5 #different isolated fragments in total flavones of Clinopodium chinense could increase cell viability.Compared to control group,SOD,GSH-Px,CAT were dropped remarkably,and LDH and MDA increased remarkably in H/R group.Compared to H/R group,2#、3#、5#medication groups decreased LDH and MDA signif-icantly,and increased SOD,GSH-Px,CAT significantly in a dose-dependent manner.Conclusion：2#、3#、5#can reduce myocar-dial damage induced by hypoxia/reoxygenation,the mechanism is related with the enhancement ability of myocardial cells to clear oxygen free radicals,and decrease the generation of lipid peroxide.
出处
《世界中医药》
CAS
2015年第8期1169-1172,共4页
World Chinese Medicine
基金
国家自然科学基金项目(编号:81374010)
国家自然科学基金项目(编号:81173511)
关键词
风轮菜总黄酮
H9C2
心肌细胞
缺氧/复氧
抗氧化作用
Total Flavones of Clinopodium chinense
H9c2 myocardial cell
Hypoxia/Reoxygenation
Antioxidant
