摘要
目的探讨PTEN、p53和上皮生长因子受体(epidermal growth factor receptor,EGFR)在乳腺癌中表达的临床病理意义及其相关性。方法采用免疫组化Max Vision两步法检测209例乳腺浸润性导管癌和40例良性乳腺病变中PTEN、p53、EGFR蛋白的表达。结果 PTEN、p53和EGFR在乳腺癌中的过表达率为57.9%、55.0%和38.3%,与良性乳腺病变间差异有显著性(P均<0.05)。PTEN、p53及EGFR蛋白表达均与肿瘤大小、组织学分级、淋巴结转移、ER和PR状态、分子分型有显著相关性(P均<0.05);PTEN、p53表达与TNM分期有显著相关性;PTEN、EGFR表达与HER-2状态有显著相关性。EGFR、p53表达与PTEN呈显著负相关(P均<0.01),p53表达与EGFR呈显著正相关(P=0.000)。PTEN、EGFR表达与乳腺癌预后相关,但不是独立的预后因子。具有PTEN^-/p53^+/EGFR^+组合表达的乳腺癌生存率低于其他组合(P=0.008)。结论 PTEN低或失表达、p53突变和EGFR过表达可能协同参与乳腺癌的发生、发展,三者联合检测对乳腺癌的治疗和预后可能具有指导作用。
Purpose To study clinical pathological significance of the expression of PTEN, p53 and epidermal growth factor receptor （ EGFR） in breast carcinoma and their correlation. Methods Immunohistochemical MaxVision method was used to detect the expression of PTEN, p53, EGFR in 209 cases of infiltrating ductal carcinoma of breast and 40 cases of benign breast diseases. Results The over-expression rate of PTEN, p53 and EGFR protein in breast carcinoma was 57. 9%, 55. 0% and 38. 3% respectively, which was significantly different from those in benign breast diseases （P〈0. 05）. The expression of PTEN, p53 and EGFR in breast cancer was correlated with tumor size, histological grade, lymph node metastasis, ER status, PR status and molecular subtype （P〈0. 05）. There was an association between PTEN or p53 and TNM stage, PTEN or EGFR and HER-2 status. There was a negative correlation in the protein expression of PTEN vs EGFR or PTEN vs p53 （P〈0. 01）. There was a positive correlation between EGFR and p53 protein expression （P=0. 000）. The expression of PTEN or EGFR was correlated with the prognosis of breast cancer, but not independent prog-nostic factors. The survival rate of patients with PTEN^- /p53 ^＋ /EGFR^ was lower than those with other combined expression of PTEN/p53/EGFR （P=0. 008）. Conclusions Low or loss expression of PTEN, p53 mutation and EGFR over-expression may be coordinately involved in the pathogenesis and progression of breast cancer. The combined detection of these markers may play an important role in making treatment and indicating prognosis for breast cancer patients.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2015年第9期986-990,共5页
Chinese Journal of Clinical and Experimental Pathology
基金
山东省科技发展计划政策引导类项目(2013YD18032)
