摘要
目的研究稀释基质对尿碘检测的影响。方法取两组高碘浓度的尿液,分别在300~600和600~1 200μg/L范围,先用高尿碘（碘浓度≥300μg/L）方法检测尿碘浓度,并将结果作为对照值;然后分别用水和低碘浓度的尿作为稀释剂,将两组尿均稀释到4~300μg/L范围内,然后用过硫酸铵消化-砷铈催化分光光度法检测尿碘浓度。结果对于碘浓度为300~600μg/L的尿液,与高尿碘方法测定结果相比,用水作为稀释剂将尿稀释2倍的尿碘检测结果明显偏高（P〈0.05）,用低碘浓度尿作为稀释剂将尿稀释2倍的尿碘检测结果无明显差异（P〉0.05）;且用水稀释2倍方法的尿碘结果高于低碘浓度尿稀释2倍方法（P〈0.05）。对于浓度为600~1 200μg/L的尿液,与高尿碘方法测定结果相比,用低碘浓度尿和水梯度稀释4倍后的尿碘检测结果均较高（P〈0.05）;用低碘浓度尿梯度稀释4倍方法的尿碘结果虽也升高（P〈0.05）,但低于用水稀释4倍方法（P〈0.05）。结论低浓度尿代替水作为稀释基质可提高高浓度尿碘检测的准确性。
Objective To explore the effects of dilution of matrix on determination iodine in urine. Methods Two groups of urine samples with iodine concentration of 300-600 μg/L and 600-1 200 μg/L were determined by methods for urine with high iodine concecntration firstly,and then the samples were diluted to 4-300 μg/L by water and urine sample with low iodine concentration. After dilution,the sample was determined by As3 ＋-Ce4 ＋catalytic spectrophotometry using ammonium persulfate digestion,and the results were reported and compared. Results For the urine samples with iodine concentration of 300-600μg/L, compared with the methods for urine with high iodine concentration,the concentrations determined by two fold dilution with water were higher apparently（P〈0.05）, but the concentrations determined by two fold dilution with urine had no significant difference. The concentrations determined by two fold dilution with water were higher than those by two fold dilution with urine（P〈0.05）. For the urine samples with iodine concentrations of 600-1 200 μg/L, compared with the methods for urine with high iodine concentration,the concentrations determined by four fold dilution with water and urine were both higher（ P〈0.05）,but the concentrations determined by four fold dilution with urine were lower apparently（ P〈0.05）. Conclusion It＇s more accurately to determine higher iodine in urine by dilution with low-iodine urine instead of water.
出处
《环境与健康杂志》
CAS
北大核心
2015年第7期629-631,共3页
Journal of Environment and Health
基金
天津医科大学大学生学术研究资助计划(2014)
天津医科大学基础医学院大学生创新基金(2014)
关键词
高尿碘
稀释基质
检测
High iodine in urine
Dilution of matrix
Determination
