摘要
目的增殖诱导配体(APRIL)与结直肠癌细胞对5-氟尿嘧啶(5-FU)的耐药性密切相关。我们前期已利用噬菌体随机肽库技术成功筛选并人工合成与s APRIL有高亲和力的结合肽。本研究旨在探讨s APRIL结合肽对结直肠癌LOVO细胞5-FU化疗敏感性的影响。方法将LOVO细胞分为PBS组、5-FU组、s APRIL结合肽+5-FU组。采用CCK-8法检测细胞增殖、流式细胞仪测定细胞周期及细胞凋亡。结果 5-FU组和s APRIL结合肽+5-FU组增殖抑制率分别为(37.62±7.07)%、(63.05±2.15)%;流式细胞仪检测各组的细胞周期显示5-FU组阻滞在S期,s APRIL结合肽+5-FU组阻滞在G0/G1期和S期,且S期阻滞效应显著强于5-FU组;三组细胞凋亡率分别为(0.21±0.09)%、(12.80±0.30)%、(17.1±0.66)%。结论 s APRIL结合肽能增强结直肠癌LOVO细胞对5-FU的化疗敏感性,有望成为结直肠癌靶向治疗的新型候选制剂。
Objective This study aimed to verify if the sAPRIL-binding peptide can enhance chemo-sensitivity of 5-FU on colorectal cancer cells. Methods LOVO cells were divided into three groups： PBS, 5-FU, and sAPRIL-binding peptide plus 5-FU. The cell growth inhibition rate was detected by CCK-8 assay, and the cell cycle and cell apoptosis were detected by flow cytometry. Results The cell growth inhibition rate was （0±0）%in PBS group, （37.62±7.07）%in 5-FUgroup, （63.05±2.15）%in sAPRIL-binding peptide plus 5-FU group, respectively. In 5-FU group, cell cycle was arrested at S phase;and in sAPRIL-binding peptide plus 5-FU group, in sAPRIL-binding peptide plus 5-FU group, cell cycle was arrested at both G0/G1 and S phases （ more markedly at S phase compared with 5-FU alone）. The percentage of early apoptotic cells was （0.21±0.09）%in PBS group, （12.80±0.30）%in 5-FUgroup, and （17.1±0.66）%in sAPRIL-binding peptide plus 5-FU group, respectively. Conclusions The sAPRIL-binding peptide can enhance chemo-sensitivity of 5-FU on colorectal cancer LOVO cells. This peptide might be a new approach for molecular targeted therapy of colorectal cancer.
出处
《现代消化及介入诊疗》
2015年第4期336-338,共3页
Modern Interventional Diagnosis and Treatment in Gastroenterology
关键词
结直肠癌
增殖诱导配体
5-氟尿嘧啶
细胞增殖
细胞凋亡
Colorectal cancer
A proliferation-inducing ligand
5-fluorouracil
Cell proliferation
Cell apoptosis
