摘要
目的探讨黄芪对弓形虫wx2b2a表位疫苗免疫小鼠后抗急性弓形虫感染的免疫保护作用。方法将小鼠随机分成pcDNA3-W2b2a组、pcDNA3-W2b2a+黄芪治疗组、pcDNA3+黄芪治疗组、黄芪治疗组、pcDNA3及生理盐水对照组。将弓形虫pcDNA3-W2b2a肌注免疫pcDNA3-W2b2a组、pcDNA3-W2b2a+黄芪治疗组小鼠3次,末次免疫后4周,每组小鼠腹腔注射102个速殖子,感染后2d起,pcDNA3-W2b2a+黄芪治疗组、pcDNA3+黄芪治疗组及黄芪治疗组小鼠给予黄芪75mg/d灌胃治疗,连续用药7d,用ELISA法测定免疫前、末次免疫后4周、感染后6d小鼠血清IgG水平及免疫前、末次免疫后2周、感染后4、6、8d小鼠IFN-γ、IL-18水平,并观察弓形虫感染后小鼠的生存时间。结果 pcDNA3-W2b2a免疫小鼠后血清IgG抗体水平均高于其它组(P<0.05),末次免疫后4周,感染后第6dpcDNA3-W2b2a、pcDNA3-W2b2a+黄芪治疗组小鼠特异性IgG抗体水平无显著性差异(P>0.05);末次免疫后2周、感染后4d、6d、8dpcDNA3-W2b2a+黄芪治疗组小鼠血清IFN-γ水平分别高于其它组(P<0.05);感染后各组小鼠血清IL-18水平持续上升,但感染后8d,黄芪治疗后小鼠血清IL-18水平显著低于pcDNA3和生理盐水对照组(P<0.05);小鼠RH株速殖子感染后,pcDNA3-W2b2a+黄芪治疗组小鼠存活时间明显长于pcDNA3-W2b2a组、pcDNA3+黄芪治疗组及黄芪治疗组(P<0.05)。结论黄芪可增强弓形虫wx2b2a表位疫苗免疫小鼠后抗急性弓形虫感染的免疫调节作用。
We studied the immunomodulatory effect of Astragalus on immune mice from the epitope vaccines wx2b2a of Toacoplasrna gondii （T. gondii） against acute T. gondii infection. The mice were randomly divided into Group pcDNA3 W2b2a, Group pcDNA3-W2b2a ＋ Astragalus treatment, Group pcDNA3 ＋ Astragalus treatment, Group Astragalus treat- ment, and Group pcDNA3 and physiological saline. The mice in Group pcDNA3-W2b2a and Group pcDNA3-W2b2a ＋As- tragalus treatment were intramuscularly immunized with pcDNA3-W2b2a of T. gondii for three times. Four weeks after the last immunization, the mice in each group were intraperitoneally injected with 102 tachyzoites. Starting from 2 days after infec- tion, the mice in Group pcDNA3-W2b2a ＋Astragalus treatment, Group pcDNA3 ＋Astragalus treatment, Group Astragalus treatment were orally treated with Astragalus with 75 mg/d for 7 days. ELISA method was taken to assay the serum levels of IgG before immunization, 4 weeks after the last immunization and 6 day post-infection （dpi） , and the levels of IFN-γ and IL 18 before immunization, 2 weeks after the last immunization and 4, 6 and 8 dpi, as well as to observe the survival time of the mice attacked by T. gondii. The serum levels of IgG of the mice which were immune with pcDNA3-W2b2a were higher than those in other groups （P〈0.05）. The levels of IgG antibodies in Group pcDNA3-W2b2a, Group pcDNA3-W2b2a ＋ Astraga lus treatment were almost the same in four weeks after the last immunization and 6 dpi （P〉0.05）. The levels of IFN-γ in Group pcDNA3-W2b2a ＋ Astragalus treatment were higher than those in other groups （P〈0.05） in 2 weeks after the last immunization and 4, 6, 8 dpi. The serum levels of IL-18 kept elevating after infection. But they are significantly lower than those in Group pcDNA3 and physiological saline after 8 days with Astragalus treatment （P〈0.05）. After infection with RH tachyzoites, the mice in Group pcDNA3-W2b2a ＋ As- tragalus treatment lived longer than those in Group pcDNA3-W2h2a, Group pcDNA3 ＋ Astragalus treatment, and Group As- tragalus treatment （P〈0.05）. Astragalus can strengthen the immunomodulatory effect on immune mice from the epitope vac- cines wx2b2a of T. gondii against acute T. gondii infection.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2015年第9期822-825,830,共5页
Chinese Journal of Zoonoses
基金
湖南省林业科技创新专项资金(No.XLK201432)
湖南省科技计划项目(No.2014FJ3126)
衡阳市社会科学基金(No.2014D100)
衡阳市科学技术发展计划项目(No.2014KJ27)联合资助~~
关键词
弓形虫
表位疫苗
黄芪
免疫调节
Toxoplasma gondii
epitope vaccine
Astragalus
immunomodulatory
