摘要
小麦的加工品质与低分子量谷蛋白亚基(LMW-GS)组成息息相关。为了尽快改良黄淮麦区小麦加工品质,应用STS分子标记与SDS-PAGE相结合的方法对小麦低分子量谷蛋白亚基Glu-A3、Glu-B3与Glu-D3位点的等位基因变异类型进行检测。结果表明:黄淮麦区南片356份小麦品种(系)中共检测到15种Glu-3位点等位变异,Glu-A3位点含Glu-A3a、Glu-A3b、Glu-A3c和Glu-A3d共4种等位变异,其分布频率分别为12.1%,13.5%,41.0%,33.4%;GluB3位点含Glu-B3a、Glu-B3b、Glu-B3d、Glu-B3f、Glu-B3g、Glu-B3h、Glu-B3i和Glu-B3j共8种等位变异,其分布频率分别为8.71%,8.99%,23.0%,5.90%,7.30%,3.65%,0.28%,42.1%;Glu-D3位点含Glu-D3a、Glu-D3b和Glu-D3c共3种等位变异,其分布频率分别为40.2%,29.8%,30.0%。等位变异组合Glu-A3c/Glu-B3j/Glu-D3a分布频率最高(10.7%)。通过优质亚基的转育,多个优质亚基的聚合,将有助于改良我国小麦的品质。
Low-molecular-weight glutenin subunits （LMW-GS ） are closely related to processing quality of wheat.The alleles on Glu-A3,Glu-B3 and Glu-D3 loci of low-molecular-weight glutenin subunit in 356 wheat vari-ties（lines）from South Yellow and Huai Valley Facultative Wheat region in China were detected by STS molecular markers and SDS-PAGE to improve wheat quality.It turned out that：There were 1 5 kinds of allelic variations in 356 detected varieties.The frequencies of the allelic variations of Glu-A3a,Glu-A3b,Glu-A3c and Glu-A3d at Glu-A3 locus were 1 2.1 %,1 3.5%,41 .0%,33.4%,respectively;the alleles of Glu-B3a,Glu-B3b,Glu-B3d,Glu-B3f,Glu-B3g,Glu-B3h,Glu-B3i and Glu-B3j at Glu-B3 locus were 8.71 %,8.99%,23.0%,5.90%,7.30%,3.65%, 0.28%,42.1 %,respectively;and the alleles of Glu-D3a,Glu-D3b and Glu-D3c at Glu-D3 locus were 40.2%, 29.8%,30.0%,respectively.The highest frequency of the subunit combination at Glu-3 was Glu-A3c /Glu-B3j /Glu-D3a （1 0.7%）.Transformating and pyramiding superior subunits can be helpful in Chinese wheat quality im-provement.
出处
《华北农学报》
CSCD
北大核心
2015年第4期25-30,共6页
Acta Agriculturae Boreali-Sinica
基金
国家“863”计划项目(2011AA10106)
陕西省自然科学基金项目(2012JM3003)
公益性行业(农业)科研专项(201303002)
西北农林科技大学唐仲英基金项目(A212021204)
