摘要
胚挽救技术是目前克服果树杂种胚早期败育的有效途径,为了探明‘黄河’‘金太阳’两个杏品种幼胚培养成苗的基本方法,以两种杏不同发育期的幼胚为试验材料,利用组织培养的方法研究了胚龄、基本培养基、植物生长调节剂等因素对幼胚萌发、成苗的影响.结果表明:基因型、胚发育指数(PF)、培养基均显著影响幼胚萌发与成苗.不同品种均随PF值的增大幼胚萌发率上升;‘黄河’杏在PF值为0.92时,萌发率最高,达61.90%;‘金太阳’杏在PF值为0.95时,萌发率最高,达71.00%.不同品种幼胚萌发的最佳培养基不同,‘黄河’杏胚萌发的最佳培养基为MS+6-BA 1.0mg/L+IAA 0.1mg/L+GA 2.0mg/L,萌发率达55.67%;‘金太阳’杏胚萌发的最佳培养基为1/2MS+6-BA 0.5mg/L+IAA 0.1mg/L,萌发率达88.67%;‘黄河’杏继代培养在MS+6-BA 0.2 mg/L+IAA 0.5mg/L培养基上长势好,成苗率达75.00%;‘金太阳’杏继代培养在MS+6-BA 0.25 mg/L+IAA 0.1mg/L培养基上长势好,成苗率达44.44%;二种杏胚继代培养形成的嫩梢在1/3MS培养基上的生根率比MS及1/2 MS培养基上的生根率高.
Embryo rescue techniques are the effective ways to overcome the fruit tree hybrid embryos early abortive. In order to find out basic methods of immature embryo culture into seedlings, two apricot varieties （cvs. ＇Yellow River＇ and ＇Golden Sun＇） with different developmental stages of young embryo was used as materials. The influence of embryo age, the basic medium and plant growth regulator was stud- ied on young embryo germination and seedling. The results shov~,ed^that the genotype,embryo development index （PF）, medium significantly affected young embryo germination and seedling. Different varieties along with the increase of PF value in the young embryo germination rate. PF value of ＇Yellow River＇ apricot at 0. 92 had the highest germination rate,with 61.90%. PF value of ＇Golden Sun＇ apricot at 0. 95 had the highest germination rate, with 71.00 %. The optimum medium for young embryo getmiriation ＇Yellow Riv- er~ was MS＋6 - BA 1.0 mg/Lq-0. 1 mg/Lq-IAA GA 2.0 mg/L,germination rate was 55. 67%. The＇opti- mum medium for young embryo germination ＂Golden Sun＂ was 1/2 MS＋6 -BA 0. 5 mg/L-I--IAA 0. 1 mg/L, germination rate was 88. 67%. The optimum transgenerational medium for ＇ Yellow RiVer＇ was MS＋6 -BA 0. 2 mg/L＋IAA 0. 5 mg/L, seedling rate was 75. 00%. The optimum transgenerational medium for ＇Golden Sun＇ was MSq-6-BA 0.25 mg/L＋ IAA 0. 1 mg/L, seedling rate was 44.44%. Two apricot germ,The rooting rate of young shoots in 1/3 MS medium was higher than that in 1/2 MS medium.
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2015年第4期69-73,共5页
Journal of Gansu Agricultural University
基金
甘肃省教育厅基本科研业务项目(035-041032)
甘肃省生物技术专项(039-034049)
关键词
杏
幼胚
萌发
继代
胚培养
apricot
young embryo
germination transgenerational
embryo culture
