摘要
目的建立一种快速、灵敏、特异的鉴定幽门螺杆菌实时荧光定量PCR方法。方法利用SYBR Green实时荧光定量PCR反应体系对口腔幽门螺杆菌进行检测。鉴定结果与临床常规鉴定方法相比较,评价其敏感度、特异度及重复性。结果通过48例样品的检测,结果显示实时荧光定量PCR法检测标本的鉴定结果与常规PCR鉴定方法的结果对比,特异度为100%,敏感度为100%;最小能检测到102个拷贝数的重组质粒;批内重复试验和批间重复试验结果均与常规鉴定方法结果相符。结论实时荧光定量PCR法鉴定口腔幽门螺杆菌,特异度和敏感度高,重复性好,且快速、简便。该方法有望成为检测口腔幽门螺杆菌感染的一种快速有效的方法。
Objective To develop a real-time fluorescent quantitative PCR system in identification of helicobacter pylori （ H.pylori） with characteristics of celerity, sensitivity and specificity.Methods SYBR Green was used in detection of o-ral H.pylori in the real-time fluorescent quantitative PCR system.The results from the system were compared with that from clinical routine detection methods, which could be applied for evaluating sensitivity, specificity and repeatability for the system.Results The data collected from 48 samples revealed that both sensitivity and specificity of real-time fluorescent quantitative PCR are 100%;This system could detect a minimum of 102 copies plasmids;both results from within group re-peating experiments and between groups repeating experiments were corresponding with the data from traditional methods. Conclusion Real-time fluorescent quantitative PCR system has a high sensitivity, high specificity and good repeatability in quantitative determination of oral H.pylori, which is also fast and easy to operate.There fore, this approach will be used to screen oral H.pylori.
出处
《微生物学免疫学进展》
2015年第6期30-35,共6页
Progress In Microbiology and Immunology
基金
甘肃省科技重大专项计划(1302FKDA034)
