摘要
目的研究南通地区健康人群白细胞介素10(IL-10)-592位点基因多态性与血清水平的关系。方法选择在南通大学附属医院体检中心健康体检的汉族成人100名,采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术扩增目的基因片段,并用特异性限制性内切酶对所获得片段进行酶切,对研究对象IL-10-592A/C基因型进行检测并作统计学检验。应用酶联免疫吸附法(ELISA)测定受试人员血清IL-10水平。结果南通地区汉族人群中存在IL-10-592A/C基因多态性,分别为AA型55例、AC型35例和CC型10例,各基因型表达水平明显不同,AA型IL-10水平为(11.78±5.40)ng/L,CC+AC型为(18.64±9.73)ng/L(因CC型样本太少,将CC型与AC型合并),两组表达水平差异有统计学意义(P<0.01)。结论 IL-10-592A/C基因多态性影响IL-10血清水平。
Objective To study polymorphism of interleukin 10（IL-10）-592 site and its relation with IL-10 serum concentration in normal Han Chinese population. Methods A total of 100 Han Chinese adults taking physical examination in Hospital of Nantong University were selected, genotyping of IL-10-592A/C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis（PCR-RFLP） followed by statistical tests. The serum concentration of IL-10 was determined by enzyme-linked immunosorbent assay （ELISA）. Results IL-10-592A/C showed genetic polymorphism among Han population in Nantong.. 55 subjects were genotype AA, 35 subjects were genotype AC; 10 subjects were genotype CC. The concentration of IL-10 varied significantly ,which was（ll. 784-5.40） ng/L for AA genotype and （18.64±9.73）ng/L for CC and AC genotypes, with statistical difference（P〈0.01）. Conclusion IL-10-592A/C polymorphism was associated with serum concentration of IL-10.
出处
《江苏预防医学》
CAS
2015年第5期8-10,共3页
Jiangsu Journal of Preventive Medicine
基金
江苏省"六大人才高峰"(2009046)
"333人才工程"(BRA2014181)
南通市科技局(HS2014040)资助
