摘要
目的:建立有瓜石斛中原儿茶酸的含量测定方法。方法:采用高效液相色谱(HPLC)法。色谱柱为Zorbax SB-Aq色谱柱;流动相为乙腈-0.1%磷酸溶液;检测波长为220nm;流速为1.0m L·min-1;柱温为30℃。结果:原儿茶酸达到基线分离,线性关系良好(r=1.0000);平均加样回收率为101.71%,RSD为2.48%(n=6)。12批市售样品含量在0.0374~0.203mg·g^-1范围内,差异相对较大。结论:该方法灵敏,可靠,可用作有瓜石斛药材的质量评价指标之一。
Objective:To establish a method for determintation of protocatecbuic acid in Herba flickingeriae by HPLC. Methods:HPLC method was used with the Zorbax SB-Aq column, the acetonitrile-0.1% phosphoric acid was employed as a mobile phase, detection wavelength was 220nm. Column temperature was 30℃, and flow rate was 1.0mL·min^-1. Results:Baseline separation was obtained and linear relationship was good (r=-1.0000) for protocatechuic acid content. The average recovery was 101.71% and RSD was 2.48%(n=6). The content of protocatechuic acid in the 12 samples of commercially available Herba flickingeriae were among 0.0374~0.203mg·g^-1, with distinct difference. Conclusion :The method is sensitive, reliable and available for the quality control of Herba flickingeriae.
出处
《北方药学》
2015年第10期1-2,共2页
Journal of North Pharmacy