摘要
目的:通过大鼠皮肤移植急性排斥模型与正常生理稳态大鼠比较,探讨TLR4、9、10在脾脏中的表达特点及意义。方法:供体分别为6只Wistar和SD大鼠,24只受体SD大鼠随机分为四组,每组6只,取供体大鼠背部皮片移植到受体,A组:为同种异基因组,Wistar大鼠皮肤移植到SD大鼠;B组:为同种同基因组,SD大鼠皮肤移植到SD大鼠;C组:为假手术组,行皮片自体移植;D组:为正常大鼠空白对照组。在术后第1、4、7、10、14、21天时间点取各组大鼠脾脏,对脾脏单个核细胞行实时荧光定量(real-time PCR)检测TLR4、9、10的mRNA表达以及脾脏组织免疫组化检测TLR4、9、10的蛋白表达情况。结果:A组TLR4、9、10的mRNA表达明显高于B、C组,差异有统计学意义(P值<0.05);B组与C组之间的mRNA表达水平无统计学意义(P值>0.05)。TLR4、9、10蛋白在A组中的表达强呈中等阳性(++)到强阳性(+++),而在B、C、D三组中的表达基本呈现弱阳性(+)。结论:通过对大鼠皮肤移植急性排斥模型中脾脏TLR4、9、10的mRNA和蛋白表达特点进行检测,发现TLR4、9、10参与了急性排斥反应;三者之间的表达可能存在协同效应;通过抑制Toll样受体的激活可能为移植术后防治急性排斥反应发生提供治疗靶点。
Objective：The expression patterns of TLR4,9,10 in the rat spleen were compared between the rat model of acute skin-transplant rejection and physiologically normal rats ,and their physiological significance were accessed.Methods：6 Wistar and 6 SD rats were used as donors ,and another 24 SD rats were used as recipients which were divided into 4 groups of 6.The same back skin slices were transplanted from donor to recipient , Group A：allograft （ Wistar to SD ）;Group B：homograft （ SD to SD ）;Group C：autograft;and Group D：normal SD rats as control.Rat spleens were collected at 1,4,7,10,14,and 21 days after surgery ,and mRNA levels of TLR4,9,10 in splenic mononuclear cells were determined by real-time PCR and corresponding protein expression was accessed by immunohistochemistry.Results：TLR4,9,10 mRNA levels of Group A were significantly higher than that of Group B and C （ P〈0.05）;TLR4,9,10 mRNA levels showed no significant difference between Group B and C （P〉0.05）.TLR4,9,10 expressions were moderate （＋＋） to strong （＋＋＋） in Group A,and generally weak （＋） in Group B,C and D.Conclusion：The patterns of TLR4,9,10 mRNA and protein expression in the rat model of acute skin-transplant rejection indicate that the three genes are involved in acute transplant rejection;the three genes may be expressed coordinately;inhibition of the Toll-like receptors may provide therapeutic targets for acute transplant rejection.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第9期1229-1234,共6页
Chinese Journal of Immunology
基金
广东省科技计划资助项目(2009B030801365)
