摘要
将数字微镜器件加入到荧光显微成像系统中,代替传统共焦显微系统中的照明针孔,利用其调制特性,通过在数字微镜器件上加载不同图片,实现对光束的分割.对马铃薯细胞分别进行四通道、六通道、九通道的荧光细胞探测,同时采用平面反射镜作为样品,测试得到系统的深度响应曲线,分析了系统的分辨率.实验结果表明,数字微镜器件的加入实现了从点对点共焦成像变为多点并行共焦显微成像,提高了显微成像的探测速度,同时具有较高的分辨率.
Digital micromirror device is added into the fluorescence confocal microscopy system,insteads of lighting pinhole of a traditional confocal microscopy system.The modulation properties of digital micromirror device divide a light source into multiple beams through loading different pictures on digital micromirror device.Four-channel,six-channel and nine-channel fluorescence images of photo cells were detected and the system resolution was analyzed by measuring the depth response curve. The experimental results show that the microscopy system transfers from point-to-point imaging into the parallel confocal microscopy imaging based on digital micromirror device.The system provides a fast scanning speed and a high resolution.
出处
《光子学报》
EI
CAS
CSCD
北大核心
2015年第8期100-104,共5页
Acta Photonica Sinica
基金
上海市重点学科项目第三期项目(No.S30502)
上海市研究生创新基金项目(No.JWCXSL1402)
上海市人才发展基金(No.2012026)
上海市教委重点科研创新项目(No.14ZZ138)资助
关键词
显微系统
共焦
数字微镜器件
荧光细胞
并行探测
成像
深度响应
Microscopy
Confocal
Digital micromirror device
Fluorescence cell
Parallel detection
Imaging
Depth response
