摘要
目的明确脑出血后灶周脑组织不同时间点HIF-1α蛋白的表达规律,在脑水肿的发生、发展过程中的可能作用,相关性及临床意义。方法本研究以不同时间点高血压脑出血患者开颅手术中取的脑出血灶周组织为病例组标本,按发病时间分为<6 h、6~24 h、24 h^3 d、>3 d组。以手术入颅路径上远离血肿处少许破坏的的脑组织做为对照组标本,通过干湿称重法测脑含水量,同时应用免疫组化染色方法,RT-PCR方法观察各组各时间点病灶周围脑组织HIF-1α的表达变化。结果 (1)脑组织水含量的变化,脑出血后脑水肿程度随出血时间的延长逐渐加重,在出血6 h内含水量即开始增高,24 h后较明显增高,3 d左右达到峰值,之后减轻(P<0.05)。(2)脑出血血肿灶周组织中HIF-1α圴有表达,对照组与出血组相比均有显著性意义(P<0.05),且出血组各时间点间比较均有差异(P<0.05)。出血6 h后免疫阳性细胞数开始增加,24 h^3 d组阳性细胞数最多,之后逐渐下降。HIF-1α与脑水肿成正相关。结论 (1)脑出血灶周HIF-1α的表达明显升高,与脑水肿密切相关。(2)HIF-1α的异常表达在高血压脑出血后脑水肿中起重要作用,促进脑水肿的发生,为脑出血的治疗提供新的靶点。
Objective Clear To clear the cerebral hemorrhage perifocal brain tissue at different time points of HIF- 1α protein expression pattern, the possible role of the occurrence and development of cerebral edema, correlation and its clinical significance. Methods The cerebral hemorrhage foci were taken during the craniotomy operation for patients with cerebral hemorrhage at different time points. The patients were divided into 〈6 h,24 h ~ 3 d,6 -24 h and 〉 3 d groups according to the time of onset. To operative cranial path away from the hematoma at a little damage of brain tissue do as the samples in the control group, by dry wet weighing method in measuring the brain water content, and immunohistochemical staining and RT-PCR methods observe changes of brain tissue around the HIF-1 et expression in each group at different time points lesions. Results （ 1 ） The water content of brain tissue changeds, cerebral hemorrhage cerebral edema with bleeding time increased, in 6 h containing water hemorrhage increased significantly, after 24 h significantly increased, peaked in about three days, then reduced （ P 〈 0.05 ）. （ 2 ） In the human brain hemorrhage perifocal HIF-1 oLshall have expressionexpressed, as compared with the control group, hemorrhage group were significant （P 〈 0.05 ） compared to and bleeding between groups comparison showed a significant difference （ P 〈 0.05 ）. The number of immune positive cells increased after bleed- ing 6 h, and the cell numbers of 24 h ~ 3 d cells was the most, then decreased gradually. HIF-1 αwas positively related to cerebral edema. Conclusion （ 1 ）The expression of HIF-1α in the human brain was significantly higher,which was closely related to cerebral edema. （2）Abnormal expression of HIF-1α plays an important role in cerebral edema after hypertensive intracerebral hemorrhage, and promotes the occurrence of cerebral edema, and provides a new target for the treatment of cer- ebral hemorrhage.
出处
《中风与神经疾病杂志》
CAS
北大核心
2015年第9期803-807,共5页
Journal of Apoplexy and Nervous Diseases
基金
吉林省卫生厅课题(No.2012ZC017)
