摘要
目的研究荆三棱块茎抗炎有效部位(AF)对脑出血(intracerebral haemorrhage,ICH)诱导的脑损伤的保护作用。方法从小鼠尾动脉取25μL自体血直接注入纹状体,造成实验性脑出血模型;于造模后的第1天、第3天和第5天对脑出血小鼠进行神经功能缺损评分(neurological deficit score,NDS),于第3天测定脑组织含水量;应用ELISA法检测脑出血小鼠第1天、第3天和第5天脑组织匀浆中炎性细胞因子白细胞介素-6、白细胞介素-1β和肿瘤坏死因子-α的含量变化。结果 300和600 mg·kg-1荆三棱块茎抗炎有效部位都能显著减少脑出血小鼠的神经功能缺损评分,降低脑出血小鼠血肿同侧脑组织水肿的含量;在第1天和第3天明显下调脑血肿周围组织中炎性细胞因子的含量。结论荆三棱块茎抗炎有效部位对脑出血小鼠脑损伤有保护作用,其可能机制是通过下调脑出血小鼠脑组织炎性因子水平起作用。
OBJECTIVE To study the effects of anti-inflammatory active fraction （AF） from the tuber of Scirpus yagaraohwi in mice with intracerebral haemorrhage（ICH）. METHODS A 25 μL volume of autologous nonanticoagulated blood was collected from the tail artery of the mouse and then injected into the striatum using a syringe pump. Neurological deficits of the mouse was assessed 1, 3 and 5 days after ICH using a 28-point neurological deficit scale, and brain water content of the mouse cerebral tissues after ICH was measured at third day. The concentrations of pro-inflammatory cytokines in perihematoma tissues, including interleukin-6, interleukin- 1β and tumor necrosis factor-α, were measured by ELISA assay. RESULTS The results show that AF significantly reduced the neu- rological deficit scores and the brain water content after ICH. AF also markedly inhibited the levels of IL-6, IL-1β and TNF-α at first day and third day after ICH. CONCLUSION Anti-inflammatory active fraction of Scirpus yagaraohwi can improve the brain injury in mouse induced by ICH. The mechanism may involve decreased expression of pro-inflammatory cytokines of mouse after ICH.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第19期1678-1681,共4页
Chinese Pharmaceutical Journal
基金
国家自然科学基金资助项目(81173515)
江苏省高校优势学科建设工程资助项目
关键词
荆三棱
脑出血
神经功能缺损评分
脑水肿
炎性细胞因子
Scirpus yagaraohwi
intracerebral haemorrhage
neurological deficit score
brain water content
pro-inflammatory cy-tokines
