摘要
目的探讨金松双黄酮联用紫杉醇对A549细胞caspase-3蛋白及Bcl-2、Bax mRNA表达的作用。方法实验分为对照组、紫杉醇组(浓度1μg·L-1)、金松双黄酮+紫杉醇组(浓度分别为0.5,1.0μg·L-1),在药物作用72 h后,用相差显微镜观察A549细胞形态学变化;逆转录-聚合酶链反应检测细胞中Bcl-2、Bax mRNA水平;蛋白免疫印迹法检测caspase-3蛋白的表达。结果与紫杉醇组相比,联用金松双黄酮组较多细胞出现细胞核浓缩、碎裂的凋亡特征,且caspase-3蛋白及Bax mRNA表达上调而Bcl-2mRNA表达下降。结论联用金松双黄酮可增强紫杉醇对A549细胞凋亡作用,可能与调节Bc1-2、Bax及caspase-3的表达水平有关。
Objective To discuss the influence of Sciadopitysin combined with paclitaxel on the expression of caspase- 3 protein and Bcl- 2,Bax genes in A549 cells. Methods The experiment was divided into three groups： control,paclitaxel and sciadopitysin ＋ paclitaxel groups. After treatment 72 h,the morphologic change was observed by phase contrast microscope. The mRNA expression of Bcl- 2 and Bax was detected by reverse transcription- PCR. The expression of caspase- 3protein was analyzed by Western- blot. Results Compared with other groups, more A 549 cells treated by Sciadopitysin ＋ paclitaxelare appeared apoptosis characteristics nuclear condensation and fragmentation. Compared with paclitaxel group,in Sciadopitysin ＋ paclitaxelare group,the mRNA level of Bcl- 2 was down- regulated,while the protein level of caspase- 3 and mRNA level of Bax were up- regulated.Conclusion Sciadopitysin ＋ paclitaxelare strengthened the action of paclitaxel to apoptosis of A549 cells,which might be correlated with the regulation of expressions of Bcl- 2,Bax and caspase- 3.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第19期1949-1951,共3页
The Chinese Journal of Clinical Pharmacology
基金
黑龙江省教育厅科学技术研究基金资助项目(12531834)
