摘要
为探索杏鲍菇原生质体的再生条件，探讨了再生方式、稳渗剂、培养基、菌龄及其他因素对杏鲍菇原生质体再生的影响。结果表明，5～7d菌丝制备的原生质体在以NaCl为稳渗剂的酵母粉蛋白胨再生培养基上再生率最高，可达0．9％。在制备原生质体时，低酶解温度、短酶解时间、低酶含量有利于原生质体的再生。
Effect of regenerating method,osmotic pressure stabilizer,culture medium,age of mushroom,etc.on the regeneration of Pleurotus eryngii protoplast was investigated.The results showed that the protoplasts from the 5-7days old mycelia yielded the most desirable results when cultured in a yeast peptone regeneration medium with up to 0.9% of NaCl as the osmotic stabilizer.Lowered temperature,shortened time and reduced enzyme concentration could help minimize enzymatic hydrolysis in the preparation which would benefit the protoplast regeneration.
出处
《福建农业学报》
CAS
北大核心
2015年第8期784-788,共5页
Fujian Journal of Agricultural Sciences
关键词
杏鲍菇
原生质体
再生率
Pleurotus eryngii
protoplast
regeneration rate
