摘要
目的:探讨脐带间充质干细胞向成脂细胞方向分化的潜能,为脂肪组织工程提供一种来源丰富的干细胞来源。方法:采用胰酶和胶原酶Ⅰ型联合消化法获得脐带间充质干细胞,通过免疫细胞化学法对其表面标志物进行鉴定,化学诱导方法诱导脐带间充质干细胞向成脂细胞方向分化,倒置显微镜观察其形态变化,油红O染色对诱导后的细胞进行染色。结果:胰酶和胶原酶Ⅰ联合消化法分离的细胞贴壁生长,呈现成纤维细胞形态,免疫细胞化学显示脐带间充质干细胞表达CD29、CD44和CD105,但不表达CD31、CD34和CD105,脐带间充质干细胞在成脂诱导培养基中细胞生长速度明显减慢,细胞形态转变为肥大、扁平、含有大量脂滴的脂肪细胞,油红O染色示胞浆充满了油滴空泡。结论:脐带间充质干细胞具有向成脂细胞方向分化的潜能,为脂肪组织工程提供了一种来源丰富、免疫力低和低分化的种子细胞。
Objective： To explore the adipogenic differentiation potential of umbilical cord mesenchymal stem cells and provide a rich source of stem cells for adipose tissue engineering. Methods： Trypsin and collagenaseⅠdigestion method was used to obtain the umbilical cord mesenchymal stem cells. Surface markers were identified using immunocytochemical. Chemical induction method was used to induce the adipogenic differentiation of umbilical cord mesenchymal stem cells, the morphologic changes were detected by inverted microscope, oil red O staining was used on the induced cells to check the adipogenic differentiation. Results： The cells isolated from umbilical cord using trypsin and collagenaseⅠdigestion method showed the adherent growth and fibroblast morphology. Immunocytochemistry showed that the umbilical cord mesenchymal stem cells were positive for CD29, 40 CD44 and CD105 while negative for CD31,CD34 and CD105. Umbilical cord mesenchymal stem cells grew slowly, the morphology of which were flat and hypotrophic after adipogenic induction for two weeks. They contained a large number of lipid droplets and oil red O staining showed cytoplasmic vacuoles were filled with oil. Conclusion： The umbilical cord mesenchymal stem cells had the adipogenic potential, which could provide the seeding cells with an abundant source, low immunity, and poor differentiation for Adipose tissue engineering.
出处
《现代生物医学进展》
CAS
2015年第24期4624-4626,4634,共4页
Progress in Modern Biomedicine
基金
教育部博士点基金(20090181110057)
国家自然科学基金项目(81070802)
关键词
成脂分化
化学诱导
脐带间充质干细胞
Adipogenic differentiation
Chemical induction
Umbilical cord mesenchymal stem cells
