摘要
目的:研究抑制信号转导与转录激活因子3(STAT3)表达对人前列腺癌细胞DU145生长的影响。方法:用慢病毒p FLU-EGFP STAT3 shRNA(shRNA)质粒转染人前列腺癌细胞DU145构建抑制STAT3表达的稳定细胞株作为shRNA组,转染空载体作为空白对照(mock)组,采用荧光定量聚合酶链式反应(q PCR)和免疫印迹(Western blot)方法检测STAT3的mRNA和蛋白的表达水平,用结晶紫染色法检测抑制STAT3表达对细胞生长的影响;用Matrigel细胞侵袭实验检测抑制STAT3表达对DU145细胞迁移侵袭能力的影响。结果:与mock组相比,shRNA组STAT3 mRNA和蛋白表达水平显著下降,约为mock组的15%和20%,shRNA组DU145细胞的生长数目明显减少,仅为空白对照组的41.8%;shRNA组DU145细胞对基底膜的侵袭能力降低,抑制率为mock组52.9%。结论:抑制STAT3表达可降低前列腺癌细胞生长和迁移能力。
Objective： To investigate the effect of STAT3 silencing on growth and invasion of human prostate cancer cells. Methods： Lentiviruses expressing STAT3-specific shRNA were used to infect human prostate cancer DU145 cells to generate a stable cell line as shRNA group,non-specific shRNA as mock group. STAT3 expression was assessed by q PCR at transcriptional level and Western blot at protein level. The effect of STAT3 inhibition on cell growth was determined by crystal violet staining.Matrigel invasion assay was used to evaluate the effect of STAT3 inhibition on cell invasion. Results：Compared to mock group,STAT3 expression were silenced about 15% at transcriptional level and 20%at protein level. Cell growth was significantly delayed in shRNA group,about 41. 8% of mock control.STAT3 silencing significantly impaired cell invasion ability and invaded cells were about 52. 9% of mock group. Conclusions： Inhibiting STAT3 expression hinders growth and invasion of human prostate cancer cells.
出处
《贵阳医学院学报》
CAS
2015年第11期1150-1153,共4页
Journal of Guiyang Medical College
基金
国家自然科学基金项目(No.81560482)
贵州省"2011协同创新中心"支助项目[黔教合协同创新中心(2014)06]
贵州省科技厅重大专项[黔科合计Z字(2012)4010]
贵州省科技厅计划项目[黔科合LG字(2012)009]
关键词
信号转导与转录激活因子3
前列腺癌细胞
细胞生长
细胞侵袭
signal transducer and activator of transcription
prostate cancer cells
cell growth
cell invasion
