摘要
为高效集约经营云南乡土珍贵用材树种西南桦，在云南思茅、德宏选22个优良无性系为试材，从取材、材料的处理、外植体的诱导分化、芽的增殖、生根等方面探讨了西南桦组织培养繁殖技术和方法，结果表明，西南桦无性系间增殖系数及生根率均有一定差异，适宜增殖培养基为MS＋6一BA 1．0 mg／L＋KT 0．5 mg／L，增殖系数可达3-6倍，生根培养基为1／2 MS＋IBA 1．0 mg／L＋NAA 2．0 mg／L，生根率可达94．4％。
In order to propagate and cultivate Betula alnoides,taking22 varieties selected from Simao and Dehongas samples, a study on techniques and relevant measures of tissue culture of Betula alnoides was carried out from sam-ple preparing , explant differentiation inducting, bud propagating, and root generating. The results showed that theoptimum medium for inducing bud differentiation was MS＋6-BA 1.0 mg/L＋KT 0. 5 mg/L, with propagating rate of 3to 6 times, and root medium was 1/2 MS＋IBA 1.0 mg/L＋NAA 2. 0 mg/L, with rooting rate of 94. 4 %.
出处
《西部林业科学》
CAS
2015年第5期8-12,共5页
Journal of West China Forestry Science
基金
国家林业局公益性项目(201104060)“西南桦、光皮桦优良无性系育林技术研究”
云南省林业厅推广课题“西南桦优良无性系繁育”
关键词
西南桦
无性系
组织培养
Betula alnoide
clone
tisue culture
