摘要
本文对近几年广泛应用于传统发酵乳制品微生物多样性分析的宏基因组DNA分析技术进行了综述,尤其是对基于16S r RNA基因序列的分子标记技术,如:变性梯度凝胶电泳、限制性片段长度多态性分析、末端标记限制片段长度多态性分析、单链构象多态性等以及这些方法的研究进展。
In this paper, we review metagenomic DNA analysis techniques which are widely used to detect the microbial diversity in traditional fermented dairy products with particular focus on molecular marker technologies based on 16S rRNA gene sequences, such as denaturinggradientgel electrophoresis(DGGE), restriction fragment length polymorphism (RFLP), terminal restriction fragment length polymorphism (T-RFLP) and single-stran dconformation polymorphism(SSCP). The recent progresses in the application of these methods are summarized in detail.
出处
《乳业科学与技术》
2015年第5期25-28,共4页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
"十二五"国家科技支撑计划项目(2013BAD18B01)
国家自然科学基金面上项目(31471711)