摘要
Rice flour, puparium of yellow mealworm, peanut shell and sunflower seed shell are made into the culture medium, and the optimal solid-phase fermen- tation formula for M. flavoviride is figured out. In the solid-phase fermentation, Design-Expert is applied to analyze the significance of all components on the sporu- lotion, and response surface method used to optimize the fermentation medium of M. flavoviride. The results show that puparlum of yellow mealworm is the most sig- nificant component for the sporulation; and the optimal medium formula for the fermentation of M. flavoviride： 0.25-g rice flour, 0. 14-g yellow mealworm pupari- urn, 1.00-g peanut shell, and 0.25-g sunflower seedshell. In this optimal medium, the sporulation achieves 100. 013 × 10^8 spores/mL fungal liquid, 31.7% higher than that of the initial design. Application of the optimal formula will reduce the production cost, obtain higher yield of spores, produce no pollution, and offer tech- nical support for the future large-scale production.
Rice flour, puparium of yellow mealworm, peanut shell and sunflower seed shell are made into the culture medium, and the optimal solid-phase fermen- tation formula for M. flavoviride is figured out. In the solid-phase fermentation, Design-Expert is applied to analyze the significance of all components on the sporu- lotion, and response surface method used to optimize the fermentation medium of M. flavoviride. The results show that puparlum of yellow mealworm is the most sig- nificant component for the sporulation; and the optimal medium formula for the fermentation of M. flavoviride： 0.25-g rice flour, 0. 14-g yellow mealworm pupari- urn, 1.00-g peanut shell, and 0.25-g sunflower seedshell. In this optimal medium, the sporulation achieves 100. 013 × 10^8 spores/mL fungal liquid, 31.7% higher than that of the initial design. Application of the optimal formula will reduce the production cost, obtain higher yield of spores, produce no pollution, and offer tech- nical support for the future large-scale production.
基金
Sponsored by Anhui Province Natural Science Foundation(1408085QC52)
Science and Technology Key Projects of China Tobacco Corporation(IPM-201411)
