摘要
目的探讨骨与关节化脓性感染患者病原菌分布及其耐药性,为临床合理使用抗菌药物提供依据。方法回顾性分析2010年1月-2015年1月骨外科接收治疗的骨与骨关节化脓性感染的514例患者临床资料,对患者病原菌分布及药敏试验进行分析,采用法国生物梅里埃公司生产ABI-Expression全自动微生物分析仪对菌株进行分离鉴定,药敏试验采用K-B琼脂扩散法。结果 514例感染患者共培养出病原菌627株,其中革兰阴性菌296株占47.2%,革兰阳性菌331株占52.8%;革兰阴性菌中鲍氏不动杆菌、大肠埃希菌及奇异变形菌对亚胺培南的耐药率最低,均<10.0%,革兰阳性菌中金黄色葡萄球菌和粪肠球菌对氨苄西林和青霉素的耐药率最高均为100.0%,对万古霉素和替加环素的耐药率最低均为0。结论骨与关节化脓性感染患者的主要致病菌为革兰阳性菌,临床应根据药敏试验结果选择适当的抗菌药物对其进行治疗。
OBJECTIVE To investigate distribution and drug resistance of pathogens causing pyogenic infections of bone and joint in patients so as to provide references for clinical rational use of antibiotics .METHODS A retrospective analysis was conducted to 514 cases with pyogenic infections of bone and joint who received treatment in orthopedic surgery from Jan .2010 to Jan 2015 .Distribution of pathogens and drug susceptibility were ana‐lyzed .ABI‐expression produced by BioMerieux was adopted to isolate and identify the bacteria strains .K‐B agar diffusion method was used to detect drug sensitivity .RESULTS Totally 627 strains of pathogens were isolated from 514 cases ,including 296 strains of gram‐negative bacteria ,accounting for 47 .2% and 331 strains of gram‐positive bacteria ,holding 52 .8% .Among gram‐negative bacteria ,Acinetobacterbaumannii ,Escherichiacoli and Myceto‐zoan were the most sensitive to imipenem with the resistance rate less than 10 .0% .Among gram‐positive bacteria , Staphylococcus aureus and Enterococcus f aecalis were totally resistant to ampicillin and penicillin ,but totally sen‐sitive to vancomycin and tigecycline .CONCLUSION Gram‐positive bacteria are the main reason causing pyogenic in‐fections of bone and joint .Clinical use of antibiotics should be in accordance with the results of drug sensitivity test .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第20期4667-4669,共3页
Chinese Journal of Nosocomiology
基金
青海省科技厅基金资助项目(2014-ZJ-746)
关键词
骨与关节
化脓性感染
病原菌
耐药性
Bone and joint
Pyogenic infection
Pathogen
Drug resistance
