摘要
目的通过观察不同阶段各实验组小鼠血清中免疫球蛋白(Ig E)及肺组织中超氧化物歧化酶(SOD)活力与抑制率的变化,探讨越婢加半夏汤对过敏性哮喘小鼠的作用机制。方法随机将健康昆明种小白鼠分为正常对照组、哮喘模型组、地塞米松干预组和中药制剂组。采用卵白蛋白(OVA)、氢氧化铝凝胶混悬液腹腔注射致敏和雾化激发的方法建立小鼠哮喘模型,造模成功后,中药制剂组给予越婢加半夏溶液1 m L/100 g。地塞米松组给予腹腔注射地塞米松1 mg/kg。正常对照组和哮喘模型组小鼠分次给予生理盐水灌胃治疗。给药30 d后处死,检测各组小鼠血清Ig E水平和肺组织SOD活力,并进行HE染色观察肺组织的病理学变化。结果中药制剂组小鼠与哮喘模型组小鼠相比,前者外周血清中Ig E含量下降(P<0.05),Ig E在2组间差异有统计学意义(P<0.05);前者肺组织匀浆中SOD活力与抑制率升高,SOD活力与抑制率在2组间差异有统计学意义(P<0.05)。结论降低过敏性哮喘小鼠血清中Ig E含量及升高小鼠肺组织SOD活力与抑制率水平,提高肺组织抗氧化能力,可能为越婢加半夏汤治疗过敏性哮喘的作用机制之一。
Objective To investigate the effect and mechanism of a Chinese traditional prescription,Yuebi Banxia decoction,on allergic asthma in mice by observing the changes of serum immunoglobulin E（ IgE） and lung tissue superoxide dismutase（ SOD） activity in the mouse model of allergic asthma. Methods Forty healthy Kunming mice were randomly divided into normal control group,asthma model group,dexamethasone intervention group and Chinese medicine preparation group. The mouse model of asthma was generated by peritoneal injection of ovalbumin（ OVA） and suspension of aluminium hydroxide. The mice of traditional Chinese medicine group received Yuebi Banxia decoction in a dose of 1 mL /100 g,daily for 30 days. The mice of dexamethasone group were given intraperitoneal injection of dexamethasone 1 mg / kg. The normal control group and asthma model group were given gastric gavage of physiological saline. After 30 days administration,the serum levels of IgE and SOD activity of lung tissue were determined,and the pathological changes of lung tissue were observed using HE staining. Results The mice of traditional Chinese medicinepreparation group showed significantly decreased serum IgE level,and significantly increased SOD activity than those of the asthma model group mice（ P〈0. 05 for both）. Conclusions Reduced serum IgE and increased SOD activity in the lung tissue of mice with allergic asthma,improving the antioxidant ability of lung tissue,may be involved in the anti-allergic asthma mechanism of the Chinese traditional prescription Yuebi Banxia decoction.
出处
《中国比较医学杂志》
CAS
北大核心
2015年第9期18-21,I0002,共5页
Chinese Journal of Comparative Medicine
基金
2013年贵州省大学生创新训练计划项目(编号:201310662008)
