摘要
目的建立一种用抑制电导离子色谱仪测定实验动物环境空气中氨的方法。方法以稀硫酸作为吸收液采集空气中的氨,使氨在吸收液中转化为铵离子,选用抑制型电导检测器,CS12A阳离子分析柱,以甲磺酸作为淋洗液,通过测定吸收液中的铵离子的含量来计算空气中氨的浓度。结果铵离子在0-1.00μg/mL范围内具有良好的线性（r=0.9991）,方法精密度高（RSD〈6.38%）,最低检出浓度为0.012mg/m^3,方法的平均回收率98.8%。结论该法操作简便,无干扰离子影响,可作为实验动物环境空气中氨的测定方法。
Objective To establish an ion chromatography（ IC） method for detection of ammonia in the environmental air of laboratory animal facilities. Methods Dilute sulfuric acid was used as the absorption liquid to collect ammonia in the air. Ammonia was converted into ammonium ion in the absorption solution. With methylene sulfonic acid as the pouring liquid,the content of ammonium ion in the absorption solution was detected,and the concentration of ammonia in the air was calculated. Results The ammonium ion showed good linearity in the range of 0 to 1 μg / mL（ r = 0. 9994）,and high precision（ RSD 〈6. 38%）. The lowest detected concentration was 0. 012 mg / m^3,and the average recovery was98. 8%. Conclusions This is a simple method,easy to operate,not interfered by other ions,and can be used to detect the ammonia in the environmental air of laboratory animal facilities.
出处
《中国比较医学杂志》
北大核心
2015年第9期70-72,共3页
Chinese Journal of Comparative Medicine
关键词
氨
离子色谱法
实验动物环境空气
Ammonia
Ion chromatography（IC）
Laboratory animal
Environmental air
