摘要
在建立同时定量麻黄汤中9个有效成分（麻黄碱、伪麻黄碱、甲基麻黄碱、苦杏仁苷、甘草苷、桂皮醇、桂皮酸、桂皮醛、甘草酸）方法的基础上,测定相应的微透析探针体外回收率,为体内药代动力学研究提供依据。HPLC-DAD检测透析液中9个有效成分浓度,考察探针回收率的日内稳定性以及流速（增量法、减量法）、媒介浓度对回收率的影响。结果 9个有效成分在52 min内可以达到良好的分离,当流速为1.0μL·min-1时,测得麻黄碱、伪麻黄碱、甲基麻黄碱、苦杏仁苷、甘草苷、桂皮醇、桂皮酸、桂皮醛、甘草酸的回收率分别为（50.95±0.82）%,（52.74±1.13）%,（51.29±0.51）%,（32.56±0.84）%,（45.36±0.83）%,（70.94±0.99）%,（69.98±2.30）%,（71.68±0.63）%,（22.14±0.48）%,且探针能在7 h内保持稳定。在相同浓度下,探针回收率随流速的增大呈指数下降,除苦杏仁苷、桂皮醛外,其余7个成分2种方法所得回收率在一定流速下相差不大。在相同流速下,桂皮醇、桂皮酸、桂皮醛回收率随浓度变化较大（9.55%~16.2%）,其余6个成分变化较小（3.27%~5.71%）。表明微透析法可用于麻黄汤中9个成分体外回收率的测定,当流速为2.0μL·min-1时反透析法可作为麻黄碱、伪麻黄碱、甲基麻黄碱、甘草苷、桂皮醇、桂皮酸和甘草酸体内探针回收率的校正方法。
To detect the in vitro probe microdialysis recoveries based on an HPLC-DAD method for simultaneous quantification of nine active ingredients（ ephedrine,pseudoephedrine,methylephedrine,amygdalin,liquiritin,cinnamyl alcohol,cinnamic acid,cinnamaldehyde and glycyrrhizic acid） in Mahuang decoction,which provides reference for in vivo pharmacokinetic study. The concentrations of nine active ingredients in dialysate were detected by HPLC-DAD,to investigate the effect of flow rates（ incremental method and subtraction method） and intraday stability of the probe recoveries and medium concentrations on the recoveries. Nine active ingredients could be well separated in 52 min. At the perfusion rate of 1. 0 μL·min- 1,the relative recoveries of ephedrine,pseudoephedrine,methylephedrine,amygdalin,liquiritin,cinnamyl alcohol,cinnamic acid,cinnamaldehyde and glycyrrhizic acid were（ 50. 95 ±0. 82） %,（ 52. 74 ± 1. 13） %,（ 51. 29 ± 0. 51） %,（ 32. 56 ± 0. 84） %,（ 45. 36 ± 0. 83） %,（ 70. 94 ± 0. 99） %,（ 69. 98 ±2. 30） %,（ 71. 68 ± 0. 63） %,and（ 22. 14 ± 0. 48） %,respectively. And the probe kept steady in 7 hours. At the same medium concentration,the probe recoveries decreased exponentially with the increase in flow rates. The recoveries of seven ingredients detected by these two methods were similar at certain flow rates,except for amygdalin and cinnamaldehyde. At the same flow rate,the relative recoveries of cinnamyl alcohol,cinnamic acid and cinnamaldehyde changed greatly（ 9. 55%-16. 2%） and the others six ingredients had less change（ 3. 27%-5. 71%） with the changes in medium concentrations. Microdialysis method could be used to detect the in vitro recoveries of nine ingredients in Mahuang decoction. Reverse dialysis method could be used for the in vivo probe recovery calibration of ephedrine,pseudoephedrine,methylephedrine,liquiritin,cinnamyl alcohol and cinnamic acid at the flow rate of 2. 0 μL·min- 1.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第18期3667-3673,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81573868)
浙江省自然科学基金项目(LR13H270001
LZ14H270001)
浙江省卫生高层次创新人才培养工程项目
