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维氏气单胞菌生物膜体外模型的建立及影响因素的研究 被引量:3

Establishment of in vitro Biofilm Model and Influence Factors of Aeramonasveronii
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摘要 为探索不同理化因素对致病性维氏气单胞菌(Aeramonasveronii,AV)‘TH0426成膜的影响,采用改良微孔板法建立致病性AV生物膜体外模型并对其进行了研究。结果表明,AVTH0426能够在聚乙烯酶标板表面形成生物膜,PH值4~6,10℃和NaCl浓度4 g/L培养18 h是形成生物膜的最适条件,黄颡鱼(Pseudobagrusfulvidraco)的肝脏提取液对AV TH0426形成生物膜有促进作用。这为进一步探讨维氏气单胞菌生物膜形成机制和致病机理提供了参考。 To study plate test was used results showed that optimum conditions and culturing for 18 tO AV for h. effect of different factors on bacterial biofilm formation in vitro, the modified microtiter - establish an in vitro biofilm model of pathogenic Aeramonasveronii TH0426 strain. The TH0426 could develop biofilm on the surface of the polystyrene microtiter plate, and the biofilm formation of AVTH0426 strain were pH 4 -6, temperature 10 ℃ , NaCI(4 g/L) , The extract of liver increased the ability of biofilm formation by testing on the AV TH0426.
出处 《中国兽药杂志》 北大核心 2015年第10期10-14,共5页 Chinese Journal of Veterinary Drug
基金 国家自然科学基金项目(31201927) 吉林省重点科技攻关项目(20150204065NY) 国家大学生创新创业训练计划(201410193023)
关键词 维氏气单胞菌 形成特性 生物膜 影响因素 Aeramonasveronii(AV) characteristics biofilm physical factor
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