摘要
目的探讨深圳市龙岗区感染性腹泻病人中沙门氏菌的感染状况及分子流行病学分析。方法 2010-2013年间从深圳市龙岗区人民医院感染性腹泻监测哨点,共采集腹泻病人粪便标本3 604份,按国标GB/T 4789.4-2013对样本进行分离鉴定,采用脉冲场凝胶电泳（PFGE）对沙门菌进行分子分型分析。结果 3 604份腹泻标本中,男女比例为1.25∶1,病例构成主要集中在0~6岁,所采样本中阳性数829例,分离出沙门菌142株,占感染性腹泻病原谱的17.13%（142/829）,检出率最高的是8-9月占34.51%（49/142）;血清分型结果表明该地区流行的沙门菌优势血清型为常见的肠炎和鼠伤寒沙门氏菌,PFGE带型表明肠炎沙门菌之间具有高度的同源性。结论深圳市龙岗区腹泻病例主要由沙门菌感染引起,肠炎沙门菌为常见的病原菌,其PFGE带型相似度高,来自同一克隆。
Objective To understand infection status and molecular epidemiological characteristics of Salmonella spp. from diarrhea patients in Longgang District of Shenzhen. Methods A total of 3 604 stool samples were collected from patients with infectious diarrhea from Longgang District People’s Hospital surveillance sites.Samples were isolated and identified according to the national standard GB / T 4789.4-2013.Molecular typing was performed by pulsed-field gel electrophoresis（PFGE） for Salmonella spp. Results The ratio of male to female was 1.25 ∶1 among 3 604 samples of infectious diarrhea.The peak incidence was occurred in 0 ~6 years of age. 142 Salmonella were isolated, with 17.13%（142 / 829）of infectious diarrhea pathogen spectrum.The peak season was August and September,34.51%（49 / 142）. Serotype results showed that S.enteritidis and S.typhimurium were the most common serotypes. PFGE patterns indicated that S.enteritidis isolates had a high similarity. Conclusions The diarrhea cases in Longgang District of Shenzhen were mainly caused by Salmonella infections. S.enteritidis was the most common pathogen. The PFGE patterns from the same clone had a high similarity.
出处
《热带医学杂志》
CAS
2015年第9期1262-1265,共4页
Journal of Tropical Medicine
基金
深圳市感染性腹泻病原谱哨点监测项目资助
