摘要
利用RACE技术从蚤状溞(Daphnia pluex)中克隆到doublesex(dpdsx),dpdsx c DNA全长为1636 bp,其中开放阅读框(ORF)1008 bp,编码335个氨基酸,含有1个DM结构域和1个Dimersation domain。同源性比对结果显示蚤状溞dpdsx与大型溞(Daphnia magna)同源性最高为77%,其次盔形溞(Daphnia galeata)为61%,模糊网纹溞(Ceriodaphnia dubia)为54%;进化分析发现蚤状溞dpdsx与盔形溞、大型溞、模糊网纹溞等溞科的亲缘关系最近;Real time-PCR实验结果表明,dpdsx在雄溞表达水平最高,其次是孤雌溞(带夏卵)和两性溞(带冬卵),雌溞与雄溞有显著性差异(P>0.05)。表明doublesex有可能在蚤状溞生殖转化和性别分化调控上起一定作用。
The full-length cDNA of a doublesex （ dpdsx） gene was cloned from the cladoceran Daphnia pulex using rapid amplification of complementary DNA ends （ RACE ） method.The dpdsx cDNA is 1636 bp in length; and it has a 1008-bp open reading frame （ ORF） that encodes a 335-amino-acid polypeptide containing a DM structural domain and a dimersation domain.Homology comparison showed Daphnia pulex′s dpdsx sharing homology of up to 77%with Daphnia magna, 61% with Daphnia magna and 54% with Ceri-odaphnia dubia.Phylogenetic analysis revealed that Daphnia pulex′s dpdsx had a close genetic relationship with Daphniidae such as D. magna, D.magna, C.dubia and so on.Real time-PCR showed that dpdsx had the highest expression level in the male, followed by the parthenogenetic female （ with summer egg） and sexual female （ with winter egg） .The expression had significant difference （ P〉0.05） between the male and the female.The experiment indicated that doublesex gene may be play a role in reproduction switching and controlling sex differentiation of D.pulex.
出处
《生物学杂志》
CAS
CSCD
2015年第5期25-29,47,共6页
Journal of Biology
基金
国家自然科学基金资助项目(31172043)
浙江省自然科学基金资助项目(LY12C19003)
宁波市创新团队项目(2011B81003)
王宽诚教育基金会资助
