摘要
目的:探讨糖肝康对糖尿病慢性肝损伤模型大鼠肝脏组织中Fas基因表达的影响。方法:用STZ和TAA建立糖尿病慢性肝损伤大鼠模型,分为正常组、模型组、护肝宁组和糖肝康小、中、大剂量组。模型组、正常组予0.9%氯化钠溶液10m L/kg灌胃;护肝宁组予0.04g/m L护肝宁悬浊液10m L/kg灌胃;糖肝康小、中、大剂量组分别予浓度1、2、4g/m L糖肝康10m L/kg灌胃。12周后,用荧光PCR测肝脏中Fas基因的表达。结果:与正常组比,模型组Fas的表达明显升高(P<0.01);与模型组比,护肝宁组和糖肝康小、中、大剂量组Fas的表达均明显减少(P<0.01,P<0.05);糖肝康大剂量组与护肝宁组比,差异显著(P<0.05)。结论:慢性肝损伤的发生发展可能与Fas基因的异常表达相关,糖肝康可通过抑制Fas基因表达水平,起到对肝脏的保护作用。
Objective：To observe and investigate the effects of Tanggankang on the expression of Fas gene in the liver of rats with chronic liver injury.Methods：Chronic liver injury rat models were established by using STZ and TAA.Rats were randomly divided into control group,model group;Huganning group,and high,middle and low dosage of Tanggankang groups.Rats in model and control groups received gavage administration with normal saline（10mL/kg）,rats in Huganning group received gavage administration with 0.04g/mL Huganning Turbid Liquid（10mL/kg）,and rats inhigh,middle and low dosage of Tanggankang groups received gavage administration with 4g/mL,2g/mL and1g/mL,Tanggankang（10mL/kg）,respectively.After treating for 12 weeks,the expression of Fas gene in liver was detected by using fluorescence PCR.Results：Compared with control group,the expression of Fas gene in liver of rats in model group was increased significantly（P〈0.01）.Compared with model group,the expression of Fas gene in liver of rats in Huganning group and all Tanggankang group were decreased significantly（P〈0.01,P〈0.05）.The difference in the expression of Fas gene between high dosage of Tanggankang group and Huganning group was significant（P〈0.05）.Conclusion：The development and progression of chronic liver injury might relate with the abnormal expression of Fas gene,and Tanggankang could play the role of protecting the liver by inhibiting the expression of Fas gene.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第10期3662-3665,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
山东省自然科学基金委面上项目(No.ZR2012HM093)
山东省中医药科学技术研究项目(No.2011-038)~~
关键词
糖肝康
慢性肝损伤
荧光PCR
FAS基因
糖尿病
Tanggankang
Chronic liver injury
Fluorescent PCR
Fas gene
Diabetes mellitus
