摘要
目的研究胰岛素样生长因子结合蛋白(IGFBP)-2及IGFBP-6在结直肠腺瘤(CRA)中的表达及临床意义。方法收集承德医学院附属医院2012年7月至2013年3月手术治疗后经病理证实为CRA 50例(CRA组)、结直肠癌(CRC)组织标本50例(CRC组),结直肠正常黏膜20例(对照组)。采用免疫组织化学法及RT-PCR方法检测IGFBP-2及IGFBP-6的蛋白及mRNA的表达情况,结合临床病理资料进行统计学分析。结果 IGFBP-2蛋白的阳性表达及mRNA的表达量在CRA组与对照组比较有升高趋势;而与CRC组比较则有降低趋势,且差异有统计学意义(P<0.05),而IGFBP-6蛋白的阳性表达在CRA组与对照组比较有降低趋势;而与CRC组比较则有升高趋势,IGFBP-6 mRNA表达量在CRA组与对照组比较有升高趋势;而与CRC组比较则有降低趋势,且差异有统计学意义(P<0.05),在CRA组,IGFBP-2、IGFBP-6的阳性表达与患者的年龄、性别、肿瘤的数量及部位差异均无统计学意义(P>0.05),但是其与增生程度差异有统计学意义(P<0.05);在CRA组,IGFBP-2与IGFBP-6的表达相互间呈负相关,差异有统计学意义(P<0.01)。结论 CRA在结直肠正常黏膜向CRC转化过程中的中间环节,而在其发生、发展过程中,胰岛素样生长因子家族(IGFs)与IGFS-R轴发挥着重要且不可替代的作用,所以IGFBP-2、IGFBP-6有可能作为CRA诊断及预后的早期预测指标。
Objective To study the insulin-like growth factor binding protein（IGFBP）-2and IGFBP-6expression and clinical significance in colorectal adenomas.Methods A collection of Chengde Medical College Hospital from July 2012 to March 2013 after surgical treatment of colorectal cancer confirmed by pathology（colorectal cancer,CRC）tissue samples of 50 patients,colorectal adenomas（colorectal adenoma,CRA）50cases,20 cases of colorectal normal mucosa.Immunohistochemistry and RT-PCR were used to detect the expression of IGFBP-2and IGFBP-6protein and mRNA,combined with clinical and pathological data were statistically analyzed.Results IGFBP-2protein positive expression and the amount of mRNA expression in the CRA group compared with normal colorectal mucosa had a rising trend;While compared with CRC group had a tendency to reduce,and the differences are obvious statistical significance（P〈0.05）,and IGFBP-6protein positive expression in the CRA group compared with normal colorectal mucosa had a lower trend;While compared with CRC group amount IGFBP-6mRNA expression in the CRA group compared with normal colorectal mucosa had a rising trend;While compared with CRC group had a tendency to reduce,and the differences were obvious statistical significance（P〈0.05）.In the CRA group,IGFBP-2,IGFBP-6positive expression and the patient′s age,sex,tumor and the number of parts were no significant statistical difference（P〉0.05）,but with the degree of hyperplasia had significant statistical difference（P〈0.05）;In the CRA group,IGFBP-2and IGFBP-6expression were negatively related to each other,and the difference was statistically significant（P〈0.01）.Conclusion Colorectal adenomas in normal colorectal mucosa of colorectal cancer to the middle part of the transformation process,and in its occurrence and development process,insulin-like growth factor family（IGFs）and IGFS-R axis plays an important and irreplaceable role,so IGFBP-2,IGFBP-6may be used as diagnostic colorectal adenomas and early predictors of prognosis,clinical studies on colorectal adenomas is important.
出处
《重庆医学》
CAS
北大核心
2015年第29期4112-4115,共4页
Chongqing medicine
关键词
结直肠腺瘤
胰岛素样生长因子结合蛋白2
胰岛素样生长因子结合蛋白6
免疫组织化学
逆转录聚合酶链反应
colorectaldenoma
insulin-like growth factor binding protein 2
insulin-like growth factor binding protein 6
im-munohistochemistry
reverse transcriptase polymerase chain reaction
