摘要
目的 从外周血单个核细胞 (PBMC)在特异性和非特异性刺激下 1型和 2型细胞因子的反应探讨宫内感染乙型肝炎病毒 (HBV)导致免疫失败的机制。方法 采用体外细胞培养和ELISA技术对 2 9例宫内感染免疫失败儿童、9例宫内感染有效儿童及 2 5名正常儿童PBMC在丝裂原 (植物血凝素和细菌脂多糖 )、酵母重组乙型肝炎表面抗原 (HBsAg)及无刺激物时干扰素 (IFN) γ、白细胞介素 (IL) 4和IL 12P70的分泌进行检测。结果 丝裂原刺激时IFN γ分泌和IFN γ自发分泌在免疫失败组有增高趋势。和自发分泌比较 ,在HBsAg刺激时 ,对照组、有效组和免疫失败组均表现为分泌增加 ,但免疫失败组增加值显著性低于对照组。在各种刺激时IL 4和IL 12分泌低于可检测水平。丝裂原刺激时IFN γ分泌和血清丙氨酸转氨酶水平正相关。结论 特异性刺激时 1型细胞因子分泌不足可能是宫内感染HBV导致乙肝疫苗免疫失败的原因之一 。
Objective To investigate the type 1 and type 2 cytokines response in children with hepatitis B (HB) immunization failure. Methods All subjects came from an HB maternal infant transmission interruption program. Children born to HB carrier mothers were immunized with at least 3 doses of HB vaccine routinely, and followed up to assess the HB virus (HBV) status. Peripheral blood mononuclear cells from 29 children with persistent HBV infection since birth who had no anti HBs response, 9 children with transient HBV infection who had HB surface antigen during the first month of life and developed anti HBs at less than 1 year of age, 25 children with positive anti HBs response and never positive for HBsAg (control) were cultured with mitogens (phytohemagglutinin, PHA and lipopolysaccharide, LPS), recombinant yeast derived HBsAg, or just the culture medium. Interferon (IFN) gamma, interleukin (IL) 4 and IL 12P70 were analyzed by ELISA in the supernatant. Results Compared with the control or transiently infected children, persistently infected children expressed a higher IFN gamma spontaneously or upon mitogens stimulation. Higher IFN gamma secretion than spontaneous secretion could be found in the 3 groups with HBsAg stimulation, but the increase in the children with persistent infection was strikingly lower than that in the normal controls. No IL 4, IL 12P70 secretion could be detected in the supernatant upon mitogen or HBsAg stimulation. A positive correlation was found between the IFN gamma secretion and blood ALT concentration.Conclusion Over expression of IFN gamma spontaneously or upon mitogens stimulation and low expression of IFN gamma upon specific stimulation existed in children in whom hepatitis B immunization failed, and this may contribute to the immunopathogenesis and persistence of HBV infection.
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2002年第8期481-484,共4页
Chinese Journal of Pediatrics
基金
卫生部临床重点学科建设项目 (970 3 0 2 2 3 )
国家自然科学基金项目 (3 9670 667)
