摘要
目的检测持续性中重度变应性鼻炎(AR)患者局部应用皮质类固醇激素治疗前后外周血中嗜酸粒细胞集落形成因子(EOS-CSF)、白介素5(IL-5)的水平,判断AR症状缓解以及临床药物治疗效果评价的方便快速的实验室指标。方法研究分AR组、AR治疗4周组、AR治疗12周组及正常对照组。采用ELAISA方法检测外周血EOS-CSF、IL-5水平,常规计数外周血嗜酸粒细胞(EOS)。应用视觉模拟量表(VAS)评价患者的鼻部症状。结果AR组血中EOS-CSF和IL-5水平及外周血中EOS数目明显高于正常对照组。AR治疗4、12周组与AR相比,IL-5和EOS-CSF水平及外周血中EOS数目都明显降低。患者血清IL-5水平与EOS计数呈正相关(r=0.63,P<0.05);血清EOS-CSF水平与EOS计数呈正相关(r=0.68,P<0.05)。结论经鼻用类固醇激素治疗后,检测外周血EOS-CSF和IL-5及查外周血EOS数目可用以监测变应性鼻炎治疗效果并对调整用药提供指导。
Objective To detect the levels of eosinophil colony stimulating factor （EOS-CSF）,interleukin-5 （IL-5 ） and eosinophil（EOS）numbers in persistent moderately and severe allergic rhinitis patients’peripheral blood and to investigate the relationship between the levels of EOS-CSF and IL-5 and the clinic symptom of AR patients after using the Mometasone Furoate Aqueous Nasal Spray.Methods The levels of EOS-CSF and IL-5 in peripheral blood serum were detected by enzyme linked immunosorbent assay （ELISA）.The number of EOS of fresh blood smear were observed by stained by Wright Giemsa method.The patients’nasal symptoms were evaluated by visual analogue scale. Results The levels of IL-5 and EOS-CSF and the EOS number in the AR group were significantly higher than in the normal control group .There was significant reduction of IL-5 and EOS-CSF in the patients after therapy.The data showed that the IL-5 level and EOS-CSF were positively correlated with EOS number.Conclusion Detection EOS-CSF,IL-5 and EOS number of peripheral blood in AR patients after treatment with nasal steroid hormone can be used to monitor the efficacy of treatment and provide guidance to adjust medication.
出处
《山东大学耳鼻喉眼学报》
CAS
2015年第5期43-46,共4页
Journal of Otolaryngology and Ophthalmology of Shandong University
基金
山东省科技攻关项目(2009gg10002025)
