摘要
目的探讨甲状腺素对生发基质-脑室内出血(GM-IVH)早产兔脑组织中甲状腺素脱碘酶表达的影响及其神经保护作用。方法将孕29天的早产兔60只随机分为对照组、模型组和干预组,模型组和干预组腹腔注射50%丙三醇建立GM-IVH动物模型,对照组给予同等剂量的生理盐水,头颅超声筛查GM-IVH发生情况。发生GM-IVH干预组早产兔腹腔注射甲状腺素,其他两组给予同等剂量生理盐水;分别于出生后第3、7和14天处死后留取脑组织。用蛋白印迹方法检测早产兔脑室周边组织中2型甲状腺素脱碘酶(D2)、3型甲状腺素脱碘酶(D3)表达;采用免疫组化方法检测并比较髓鞘磷脂碱性蛋白(MBP)表达。结果三组早产兔脑组织中D2的表达均逐渐增加;在同一时间点均为模型组最低,对照组最高,干预组介于两者之间,三组间差异均有统计学意义(P均<0.001)。对照组、模型组脑组织中D3的表达逐渐增加,干预组则以第7天时为最高;在同一时间点,均为对照组最低,第3天时干预组高于模型组,随后模型组高于干预组,三组间差异均有统计学意义(P均<0.001)。对照组、干预组MBP表达逐渐增加,模型组逐渐减少;在同一时间点均为模型组最低,对照组最高,干预组介于两者之间,差异均有统计学意义(P均<0.001)。结论甲状腺素干预在一定程度上可以使GM-IVH早产兔脑组织D2表达增加、D3表达下降,并使MBP表达增加。
Objectives To investigate the effect of thyroid hormone on the expression of deiodinase and myelin basic protein in brain tissue of neonatal rats with germinal matrix-intraventricular hemorrhage（GM-IVH）, and its nerve protective effect. Methods Sixty premature rabbits of gestational age 29 days were randomly divided into control, model and intervention groups. The premature rabbits in model and intervention groups were given intraperitoneal injection of 50% glycerol to establish GM-IVH animal model and the premature rabbits in control group were given the same dose of saline. The GM-IVH was screened by cranial ultrasound. The premature rabbits with GM-IVH in intervention group were given intraperitoneal injection of triiodothyronine and the other two groups were given the same dose of saline. At 3 days, 7 days and 14 days after birth, the premature rabbits were killed and the brain tissue were quickly separated. The expression of type 2 iodothyronine deiodinase（D2） and type 3 iodothyronine deiodinase（D3） of periventricular zone tissue were detected by western blot method. The expression of myelin basic protein（MBP） were detected by immunohistochemical method. Results The expression of D2 in brain tissue increased gradually in three groups. At the same time point, the expression of D2 was the lowest in the model group, the highest in the control group, and the middle in the intervention group. The differences of D2 among three groups were statistically significant（all P〈0.001）. The expression of D3 increased gradually in the control and model groups, while it was the highest in the intervention group at day 7. At the same time point, the expression of D3 in the control group was the lowest, it was higher in intervention group than that in the model group at 3 days, then it was higher in the model group than that in the intervention group. The differences of D3 among three groups were statistically significant（all P〈0.001）. The expression of MBP increased gradually in the control and intervention groups, and it decreased gradually in the model group. At the same time point, the MBP was the lowest in the model group, it was the highest in the control group, and it was the middle in the intervention group. The differences of MBP among three groups were statistically significant（P〈0.001）. Conclusions The expressions of D2 and MBP in the brain tissues of premature rabbit with the GM-IVH increased while the expression of D3 decreased in some extent after thyroxine intervention.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2015年第10期887-892,共6页
Journal of Clinical Pediatrics
