摘要
为探讨不同温度对血液液状保存时保存损害机制的影响和相应的防范措施 ,取 10名健康献血员静脉血 ,混合于CP2D A保存液中 ,均分为 2组 ,保存于 0℃和 4℃环境条件下 ,分别于设定的时间 (第 2 1天和第 4 2天 )以shafiq UR rehman法检测膜磷脂 ,同步法检测Na+ K+ ATP酶、纯化PDE法检测CaM、分光光度法检测LPO等指标。结果表明 ,固定温度条件下随时间的延长血液过氧化反应增强 ,保存损害作用增加 ;同一时期内保存损害作用随温度的降低而减轻 ,以 4℃组血液老化最明显。提示血液保存损害作用随保存期的延长而增强 ,随保存温度的降低而改善 ,0℃组保存血液的质量优于
To examine the effects of different temperature on preservation damages of red blood cells in liquid blood. Blood samples were taken from 10 healthy blood donors and divided each sample into 2 groups, the fresh blood was put into CP2D A solution at 0℃ and 4℃, and the samples were taken after 21 days and 42 days, and then the contents of membrane phospholipids were measured with shafiq UR rehman method, of CaM with purification PED test, of LPO with spectrophotometry. Results showed that at the same temperature, when the preservation time was prolonged, peroxidation was increased, the preservation damages were also augmented; the damages were declined when the temperature was lower during the same period, the aging of blood was more evident at 4℃. It is suggested that the 0℃ group is better than 4℃ group.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2002年第8期735-737,共3页
Medical Journal of Chinese People's Liberation Army
