摘要
目的：测定苍耳子中苍术苷的含量。方法：通过对不同产地苍耳子药材进行收集,分别对其种仁、种壳、刺、果壳进行分离处理,并测定其苍术苷含量。结果：苍耳子分离出的种仁、种壳、刺、果壳中,苍术苷标准曲线0.505~505ng/mL,具有较好内线性关系（r=0.9996）;精密度实验、重复性实验与稳定性实验结果均满足要求;加样1.5倍、1倍和0.5倍回收率分别为（92.52±1.00）%、（94.03±1.34）%、（97.62±1.96）%。结论：通过对不同产地不同部位苍耳子中苍术苷含量的测定,我们发现炮制品苍耳子种仁中苍术苷含量较高,应加强了对苍耳子种仁的炮制,以减产少毒性。
Objective： To determine the content of altractyloside in Cangerzi（ Fructus Xanthii）. Methods： By collecting Cangerzi（ Fructus Xanthii） in different producing areas,the kernels,seed-cases,stabs and shells were separated and the content of altractyloside was determined. Results： Among the separated kernels,seed-cases,stabs and shells,the standard curve of altractyloside was 0. 505 ~ 505 ng/m L,with good interior linear relation（ r = 0. 9996）. The results of precision experiment,repeatability experiment and stability experiment all met requirement. The recovery rates of 1. 5,1 and 0. 5 times adding sample were respectively（ 92. 52 ± 1. 00） %,（ 94. 03 ± 1. 34） % and（ 97. 62 ± 1. 96） %. Conclusion： This determination method is simple to conduct,with good stability and high precision and sensitivity,which can be used to effectively determine the content of altractyloside in Cangerzi（ Fructus Xanthii）.
出处
《河南中医》
2015年第9期2267-2269,共3页
Henan Traditional Chinese Medicine
基金
陕西省科学技术研究发展计划项目[编号:2006K12-G5(6)I]
关键词
苍耳子
苍术苷
毒性成分
含量测定
Cangerzi（Fructus Xanthii）
altractyloside
poisonous component
determination of content
