摘要
目的探讨败血症患者血清可溶性髓样细胞触发受体-1（sTREM-1）的表达及其对败血症的诊断价值。方法选取2013年7月~2014年12月本院收治的50例败血症患者作为败血症组,43例非败血症患者作为对照组。分别于入院第1天、治疗后住院第5天收集患者血清,采用电化学发光法测定降钙素原（PCT）、白介素6（IL-6）水平,采用定量酶联免疫吸附法（ELISA）检测sTREM-1水平。采用受试者工作特征（ROC）曲线研究sTREM-1、PCT、IL-6在败血症诊断中的效能。结果败血症组住院第1天的sTREM-1、PCT和IL-6水平显著高于对照组,差异有统计学意义（P〈0.01）。败血症组治疗前的sTREM-1、PCT和IL-6水平显著高于治疗后,差异有统计学意义（P〈0.01）。sTREM-1、PCT和IL-6诊断败血症的ROC曲线下面积（AUC）分别为0.933、0.928和0.913。sTREM-1以236.4ng/L为最佳截断点诊断败血症的敏感度为86%,特异度为91%。结论sTREM-1、PCT和IL-6诊断败血症的ROC曲线下面积均〉0.9,具有较高的诊断效能;血清sTREM-1水平可作为败血症的早期诊断指标。
Objective To explore the expression of serum soluble myeloid cells triggering receptor-1（s TREM-1） in septicemia patients and the diagnosis value for septicemia. Methods 50 patients with sepsis from July 2013 to December2014 in our hospital were selected as the septicemia group,43 patients with non septicemia were selected as the control group.Patients′ serum were respectively collected at the first day of admission and the fifth day after treatment.The level of procalcitonin（PCT） and interleukin-6（IL-6） were detected by using electrochemiluminescence.The level of s TREM-1was tested by using quantitative enzyme-linked immunosorbent method（ELISA）.The diagnosis efficacy of s TREM-1,PCT and IL-6 in the diagnosis of septicemia was studied by using the receiver-operating characteristic curve（ROC）.Results The levels of s TREM-1,PCT and IL-6 in septicemia group in the first hospitalization day was higher than that in the control group,with significant difference（P0.01）.The levels of s TREM-1,PCT and IL-6 in septicemia group before treatment was higher than that after treatment,with significant difference（P0.01）.The area of STREM-1,PCT and IL-6 in the diagnosis of sepsis under the ROC curve was 0.933,0.928 and 0.913 respectively.The optimum truncation points of s TREM-1 was 236.4 ng/L that the sensitivity and specificity was 86% and 91% respectively in the diagnosis of septicemia. Conclusion The area of STREM-1,PCT and IL-6 in the diagnosis of septicemia under the ROC curve are greater than 0.9,and have higher diagnosis efficacy.Serum levels of s TREM-1 can be used as early indicator in the diagnosis of septicemia.
出处
《中国当代医药》
2015年第28期4-7,共4页
China Modern Medicine
基金
广东省佛山市医学类科技攻关项目(201308337)
