摘要
目的:探讨斑蝥酸钠维生素B6(sodium cantharidinate and vitamin B6,SCAVB6)对人肝癌SMMC-7721细胞增殖、凋亡和侵袭及乙酰肝素酶(heparanase,HPA)表达的影响。方法:0.5、1.0、1.5和2.0μg/m L SCAVB6作用肝癌SMMC-7721细胞24、48和72 h后,应用MTT法检测细胞的增殖情况。0.5、1.0、1.5和2.0μg/m L SCAVB6作用肝癌SMMC-7721细胞48 h后,FCM法检测细胞的凋亡情况,Transwell侵袭实验检测细胞的侵袭能力,RT-PCR和蛋白质印迹法检测细胞中HPA m RNA和蛋白的表达水平。结果 :SCAVB6能够抑制SMMC-7721细胞的增殖,且具有一定的浓度和时间依赖性(P<0.05)。SCAVB6作用48 h后,肝癌SMMC-7721细胞的凋亡率高于对照组(SMMC-7721细胞未进行任何干预),而侵袭能力低于对照组(P值均<0.05)。1.0、1.5和2.0μg/m L SCAVB6作用后,SMMC-7721细胞中HPA m RNA和蛋白的表达水平低于对照组,且呈浓度依赖性(P值均<0.05)。结论 :SCAVB6可抑制SMMC-7721细胞的增殖和侵袭,这一作用可能与抑制HPA的表达有关。
Objective： To investigate the effects of sodium cantharidinate and vitamin B6 （SCAVB6） on cell proliferation, apoptosis and invasion of hepatic cancer cell line SMMC-7721 as well as the expression of heparanase （HPA）. Methods： After treatment with 0.5, 1.0, 1.5 and 2.0 μg/mL SCAVB6 for 24, 48 and 72 h, the cell proliferation was detected by MT＋ assay. The cell apoptosis, invasion ability and the expression levels of HPA mRNA and protein of SMMC-7721 cells after treatment with 0.5, 1.0, 1.5 and 2.0 μg/mL SCAVB6 for 48 h were examined by flow cytometry（FCM）, Transwell cell invasion assay, RT-PCR and Western blotting, respectively Results： SCAVB6 could inhibit the proliferation of SMMC-7721 cells in a dose- and time- dependent manner （P 〈 0.05）. The apoptotic rate of SMMC-7721 cells after treatment with SCAVB6 for 48 h was higher than that of the SMMC-7721 cells without any treatment, but the invasion ability was weakened （both P 〈 0.05）. The expression levels of HPA mRNA and protein in SMMC-7721 cells after treatment with 1.0, 1.5 and 2.0 μg/mL SCAVB6 were lower than those in the SMMC-7721 cells without any treatment, and this inhibition effect was in a dose-dependent manner （all P 〈 0.05）. Conclusion： SCAVB6 can inhibit the proliferation and invasion of SMMC-7721 cells. This effect may be related to the inhibition of expression of HPA.
出处
《肿瘤》
CAS
CSCD
北大核心
2015年第10期1092-1097,共6页
Tumor
基金
河北省中医药管理局科研计划项目(编号:2011075)~~
关键词
肝肿瘤
治疗应用
细胞增殖
斑蝥酸钠维生素B6
乙酰肝素酶
Liver neoplasms
Therapeutic uses
Cell proliferation
Sodium cantharidinateand vitamin B6
Heparanase
