摘要
目的 :观察芩莲正积方对Lewis肺癌小鼠转移的抑制作用,并探讨其机制。方法 :建立Lewis肺癌小鼠模型,芩莲正积方干预,并设对照组,观察各组抑瘤率、肺转移抑制率,采用SABC免疫组化法检测各组CD44含量、实时荧光定量PCR检测各组血管内皮生长因子(vascular endothelial growth factor,VEGF)m RNA表达情况。结果:芩莲正积方组、顺铂组及联合组的抑瘤率分别为18.16%、27.12%、36.37%,芩莲正积方可抑制肺癌转移,降低CD44、VEGF表达,与顺铂联用作用增强。结论:芩莲正积方可抑制Lewis肺癌小鼠转移,其机制可能与降低CD44、VEGF表达相关。
Objective：To observe the effects of Qinlian Zhengji formula on inhibitiing metastasis of Lewis lung cancer mouse,and to discuss its related mechanisms. Methods：Models of lewis lung cancer mouse were established for different groups of intervention. To observe groups of tumor inhibition rate and lung metasta- sis inhibition rate,test groups of CD44 content by immunohistochemical,use real-time fluorescent quantitative PCR technique to detect the expression of VEGF gene. Results：The tumor inhibition rates of Qinlian Zhengji formula group,cisplatin group and combined group were 18.16%,27.12%,36.37%,Qinlian Zhengji formula can inhibit lung cancer metastases,and reduce the expression of CD44 and vascular endothelial growth factor （VEGF） gene expression. It combined cisplatin has synergistic effect. Conclusion：Qinlian Zhengji formula can inhibit metastasis of Lewislung cancer mouse,its mechanisms may be related to its decreasing the expression of CD44 and VEGF.
出处
《山东中医杂志》
2015年第10期780-782,共3页
Shandong Journal of Traditional Chinese Medicine
基金
山东省中医药管理局课题(编号:2009Z003)
