摘要
为提升中国鳀鱼产业附加值,基于鳀鱼粗蛋白浓缩产物(鱼粉),进一步探索功能性鱼蛋白的解决方案。以鳀Engraulis ringens鱼粉为原料,采用复合酶法对其进行功能修饰,所采用的酶包括碱性蛋白酶和风味蛋白酶,对酶解产物进行离心并用硅藻土过滤,分离脱出油脂等杂质,再通过喷雾干燥后,得到浓缩的水解鱼蛋白(HFPI),对所得到的水解鱼蛋白(HFPI)进行一般理化指标和功能性分析,考察其对枯草芽孢杆菌Bacillus subtilis、保加利亚乳酸杆菌Lactobacillus bulgaricus、大肠杆菌Escherichia coli和酿酒酵母菌Saccharomyces cerevisiae等4种重要微生物的促生长作用。结果表明:经复合酶解、过滤、分离和喷雾干燥后,鳀水解鱼蛋白(HFPI)的得率可达到59.53%;其一般组成为粗蛋白质81.02%、脂肪0.52%、灰分11.52%;此水解鱼蛋白(HFPI)对上述4种菌表现出很好的促生长作用。研究表明,鳀鱼蛋白可用作开发具有微生物培养功能性的生物鱼蛋白制剂。
Functional fish protein isolate of anchovy Engraulis ringens was explored as a solution started with fish meal as a low value crude fish protein concentrate to increase value into anchovy in China. First, fish protein func-tionality of crude protein concentrate （ fish meal） was modified by hydrolysis of flavourzyme and alcalase. The hy-drolyzed fish protein was defatted, centrifuged, and filtered by diatomaceous clay, and then concentrated by spray drying to get hydrolyzed fish protein isolate （ HFPI） . Second, approximate compositions and function were analyzed in HFPI and growth-promoting effects of HFPI on 4 species of microorganisms including Bacillus subtilis, Lactoba-cillus bulgaricus, Escherichia coli, and Saccharomyces cerevisiae were evaluated. Combination of enzymatic hydroly-sis, filtration, separation and spray drying revealed that the HFPI had yield rate of 59. 53%, and contained 81. 02% crude protein, 0. 52% fat, and 11. 52% ash. It was found that HFPI showed well growth-promoting effects on microorganisms above, and was used as a functional fish protein in microorganism culture.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2015年第5期531-535,共5页
Journal of Dalian Ocean University
基金
辽宁省大学生创新创业计划大赛项目(201310158107)
国家自然科学基金资助项目(31271980)
关键词
鳀鱼
功能性鱼蛋白
水解
Engraulis ringens
functional fish protein isolate
hydrolysis
