摘要
为选择理想的琼脂及琼脂的替代物,以降低大花萱草组培苗的生产成本,收集了3种市售琼脂和1种卡拉胶,比较不同浓度的卡拉胶和琼脂对大花萱草试管苗生长的影响,降低大花萱草组培苗的生产成本。结果表明:卡拉胶对试管苗的分化生长影响表现在其分化增殖系数均高于市售的3种琼脂。卡拉胶4g·L-1为试管苗分化增殖的最佳固化剂及浓度。在试管苗生根方面,筛选出了市售琼脂3号4g·L-1为最佳生根固化剂及浓度。因此,大花萱草试管苗分化阶段固化剂采用卡拉胶4g·L-1,生根阶段固化剂采用琼脂3号4g·L-1,达到了降低生产成本的目的。
In order to choose the ideal AGAR and AGAR substitute and to reduce the production cost of Hemerocallis hybridas tissue culture seedling,the effects among three types of AGAR and a carrageenan on the tube seedings of Hemerocallis hybridas were studied.The results showed that the differentiation coefficient of tube seedlings in the carrageenan medium was bigger than them in the agar mediums.Considering solidification effect,4g·L-1 carrageenan was the best solidifying agent.The suitable medium for rooting had also selected,it was 4g·L-1 of No.3AGAR.The rooting rate and the number of the seeding root were both bigger than the other.On the tissue culture of the Hemerocallis hybridas,the solidifying agent might use 4g·L-1 carrageenan during differentiation phase,and use 4g·L-1 of No.3AGAR during rooting phase,and production cost reduced.
出处
《黑龙江农业科学》
2015年第10期24-26,共3页
Heilongjiang Agricultural Sciences
基金
河北省科技厅科技支撑资助项目(11220608D
15226406D)
关键词
卡拉胶
大花萱草
试管苗
增殖系数
生根率
carrageenan
Hemerocallis hybridas
tube seeding
differentiation coefficient
rooting rate
