摘要
【目的】大豆蚜Aphis glycines逐渐成为栽培大豆上的世界性重要害虫,为明确大豆蚜不同地理种群的遗传分化及遗传多样性,本研究探讨其在中国的种群遗传变异。【方法】测定了采自7个省共14个地理种群339头大豆蚜的线粒体COⅡ基因的673 bp序列,利用Dna SP 5.0、Arlequin 3.5.1.2、Network4.6.1.3等软件对地理种群间的大豆蚜的遗传多样性、遗传分化程度及分子变异进行分析,并建立了单倍型网络图及单倍型系统进化树。【结果】在所分析的339个COⅡ序列中,共检测出7个单倍型,其中H1为各种群所共享。种群内遗传多样性较低(Hd=0.479±0.030,Pi=0.00166±0.00018),种群内遗传分化相对较大(Fst=0.1985),基因流水平较高(Nm=2.019)。中性检验结果不显著(Tajima’s D=﹣0.931,P>0.10,Fu’s Fs=0.220,P>0.10),说明中国地区大豆蚜在较近的历史时期内没有出现种群扩张现象。分子变异分析(AMOVA)结果表明,大豆蚜遗传变异主要来自种群内部(80.15%),而种群间未发生明显的遗传分化。根据各地理种群的单倍型建立的系统发育树、单倍型网络图表明,各单倍型散布在不同的地理种群中,无明显的地理分布格局。【结论】大豆蚜不同地理种群的遗传多样性不高,各种群的遗传距离与地理距离之间没有显著线性相关性,各种群间的基因交流并未受到地理距离的影响。
[Objectives] To investigate genetic variation among different geographic populations of soybean aphids, Aphis glycines, in China. [Methods] The genetic diversity, genetic differentiation, and molecular variance of 339 individual soybean aphids from 14 geographic populations in 7 Chinese provinces were analyzed based on 673 bp segments of the mitochondrial cytochrome c oxidase subunit Ⅱ（COⅡ） gene using Dna SP 5.0, Arlequin 3.5.1.2 and Network 4.6.1.3. Phylogenetic trees and the networks of COⅡ haplotypes were constructed from the resultant data. [Results] A total of 7 haplotypes were identified; haplotype No.1（H1） was shared by all 14 populations. A low level of genetic diversity（Hd=0.479±0.030, Pi=0.00166±0.00018） in the total population was detected and higher genetic differentiation（Fst=0.1985） and a high level of gene flow（Nm=2.019） was apparent among different geographic populations. The results of a Neutral test（Tajima＇s D=﹣0.931, P0.10, Fu＇s Fs=0.220, P0.10） were not significant, indicating that there is no evidence of recent population expansion. The analysis of molecular variance（AMOVA） showed no obvious genetic differentiation among populations with a high proportion of the total genetic variance attributable to variation within populations（80.15%）. A phylogenetic tree and a haplotype network based on haplotypes of the different geographic populations shows no obvious geographical structure in different clades. [Conclusion] Genetic diversity of different geographical populations of soybean aphids is low, and there is no significant linear correlation between geographic distance and genetic differentiation; gene flow between different geographic populations is not affected by geographic distance.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2015年第5期1203-1214,共12页
Chinese Journal of Applied Entomology
基金
现代农业产业技术体系建设专项资金(CARS-04)
公益性行业(农业)专项基金(201103002)
