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纳洛酮对吗啡成瘾大鼠海马脑电功率谱的影响 被引量:2

Effects of naloxone on the hippocampal electroencephalographic power spectrum in morphine-addicted rats
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摘要 目的探讨阿片受体拮抗剂纳洛酮(naloxone,Nx)对吗啡成瘾大鼠海马脑电功率谱的影响。方法20只雄性Wistar大鼠,分为盐水组(n=10)和吗啡戒断组(rb=10),经8d生理盐水(1ml/kg)、盐酸吗啡(10mg/kg)交替皮下注射后建立躯体依赖模型,然后经盐酸纳洛酮(Nx,5mg/kg)腹腔注射观察其戒断反应;另取大鼠20只,分为盐水条件性位置偏爱(conditionedplacepreference,CPP)组(n=10)和吗啡CPP组(n=10),经8d盐水、吗啡交替注射训练建立CPP模型,然后经麻醉后注射Nx,记录Nx注射前后的背侧海马脑电,并分析功率谱数据。结果(1)与盐水戒断组比较,Nx使吗啡戒断组的戒断分数显著增加[(22.69±3.59)分VS(84.90±13.40)分,P〈0.01];(2)吗啡CPP组测试期CPP分数[(80.69±14.96)分]相较自身基础值[(45.29±15.79)分]和盐水CPP组测试期分数[(41.84±12.47)分]均显著增加(P〈0.01);(3)Nx使吗啡CPP组的海马EEG功率谱8频段功率[(81.80±30.91)mV2·Hz]较自身基线[(112.49±39.43)mV2·Hz)]、1频段功率[(25.35±11.20)mV2·Hz]较自身基线[(28.71±13.06)mV2·Hz]均可逆性的降低,差异具有统计学意义(P〈0.05)。结论Nx可降低吗啡成瘾大鼠海马脑电功率谱δ频段和γ频段功率。 Objective To investigate the effect of opiate receptor antagonist naloxone on the electroencephalographic (EEG) power spectrum in morphine-addicted rats. Methods 20 male adult Wistar rats were alternately injected with morphine ( Mf, 10 mg/kg, sc. ; Mf withdrawal (Mf-Wd) group, n= 10) or saline ( Sal, 1 ml/ kg, sc. ; Sal-Wd group, n= 10) for 8 days to establish Mf physical dependent model.Withdrawal scores were evaluated by naloxone-precipitated withdrawl signs (5 mg/kg, ip. ). Another 20 rats were divided into Sal-CPP group and Mf-CPP group with 10 rats in each group.Rats in the sal-CPP group were injected with saline once a day for 8 days while rats in the Mf-CPP group were injected with saline and morphine alternatively once a day for 8 days.The EEG signals were recorded from their dorsal hippocampi before and after naloxone injection under anesthesia; and the EEG power data (1-100 Hz) were analyzed. Results (1) Compared with Sal-Wd group (22.69±3.59),the Mf-Wd group showed a higher withdrawal scores after naloxone injection( 84.90± 13.40, P〈0.01 ). (2) The CPP scores of Mf-CPP rats (80.69± 14.96) were significantly higher than the baseline (45.29± 15.79) and the Sal-CPP rats (41.84±12.47; all P〈0.01). (3)After naloxone injection,the EEG powers ofδ band and γ band of Mf-CPP rats decreased temporarily compared with their baseline ( δ band : ( 81.80± 30.91 ) mV2 . Hz vs ( 112.49± 39.43 ) mV2 . Hz; y band:(25.35±11.20)mV2 . Hzvs (28.71±13.06)mV2 . Hz; allP〈0.05). Conclusion Opiate receptor antagonist naloxone can decrease the hippocampal EEG powers of δ band and γ band in Mr-addicted rats.
出处 《中华行为医学与脑科学杂志》 CAS CSCD 北大核心 2015年第10期869-872,共4页 Chinese Journal of Behavioral Medicine and Brain Science
基金 国家自然科学基金项目(31060142) 教育部“春晖计划”合作科研项目(Z2011126) 云南省应用基础研究计划(2011FZ084) 云南省高校民族体质健康与高原训练生物适应重点实验室项目
关键词 吗啡 纳洛酮 海马 脑电图 功率谱 Morphine Naloxone Hippocampus Electroencephalograph Power spectrum
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