摘要
目的:观察过氧化物酶体增殖物激活型受体γ(PPARγ)激动剂罗格列酮(RG)对干细胞因子(SCF)诱导的人肥大细胞迁移的影响,并探讨其机制。方法:体外培养人肥大细胞株(HMC-1),逆转录PCR方法检测HMC-1细胞上PPARγ的表达。MTT法和流式细胞术检测RG和GW9662对HMC-1细胞的细胞毒性。将HMC-1细胞分为空白对照组、SCF组、SCF+1μmol/L RG组、SCF+3μmol/L RG组、SCF+10μmol/L RG组,采用Transwell Chamber检测细胞迁移。另取HMC-1细胞分为空白对照组、SCF组、SCF+10μmol/L RG组、SCF+10μmol/L RG+0.1μmol/L GW9662组、SCF+10μmol/L RG+0.3μmol/L GW9662组、SCF+10μmol/L RG+1μmol/L GW9662组,采用Transwell Chamber检测细胞迁移。结果:HMC-1细胞株表达PPARγ,RG和GW9662对HMC-1细胞没有毒性。RG呈浓度依赖性抑制SCF诱导的HMC-1细胞的迁移。PPARγ阻断剂GW9662(0.3μmol/L及1μmol/L)可以阻断RG对HMC-1细胞迁移的抑制作用。结论:PPARγ激动剂罗格列酮通过PPARγ途径抑制SCF诱导的HMC-1细胞的迁移。
AIM： To investigate the effects and mechanism of a PPARγ ligand,rosiglitazone（ RG）,on stem cell factor（ SCF）-induced migration in human mast cell-1（ HMC-1） cells. METHODS： The mRNA expression of PPARγ was detected by Reverse Transcription（ RT）-PCR. The cytotoxic effect of RG and GW9662 on HMC-1 cells was detected by MTT and flow cytometric analysis. HMC-1 cell migration was examined with a 24-well microchemotaxis assay. HMC-1 cells were divided into control group, SCF group, SCF ＋ RG（ 1-10 μmol / L）group,SCF ＋ RG（ 10 μmol / L） ＋ GW9662（ 0-1μmol /L） group,and cells were allowed to migrate for 5 h,recovered from the lower chamber,and counted using a hemocytometer. RESULTS： HMC-1cells expressed PPARγ mRNA. RG and GW9662 had no cytotoxity on HMC-1 cells. RG inhibited SCF-induced HMC-1 cell migration in a dose-dependent manner, the selective PPARγ antagonist GW9662（ 0. 3 μmol / L and 1μmol / L） prevented the inhibitory effect of RG on HMC-1 cells. CONCLUSION： RG inhibits the migration of HMC-1 cells in a PPARγ-dependent mechanism.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2015年第9期981-984,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
