摘要
目的 探讨富氢水对6 Gy照射小鼠胸腺细胞内活性氧水平、细胞凋亡及DNA损伤程度的影响.方法 实验分为对照组、6 Gy照射组、6 Gy照射＋富氢水组;对照组和6 Gy照射组小鼠照射前10 min灌胃正常饮用水0.5 ml,6 Gy照射＋富氢水组小鼠灌富氢水0.5 ml,照射后连续灌胃,给予小鼠正常饮用水和富氢水7d,于照射后4、7、15d处死小鼠获取胸腺细胞.采用流式细胞术检测胸腺细胞内活性氧水平、凋亡细胞比例及磷酸化组蛋白H2AX（γ-H2AX）平均荧光强度.结果 与对照组的小鼠比较,6 Gy照射组小鼠在照射后4、7、15d胸腺细胞中的活性氧水平升高,早期凋亡细胞[AnnexinV阳性、碘化丙啶（PI）阴性]和晚期凋亡细胞（AnnexinV阳性、PI阳性）的细胞比例明显增加,γ-H2AX平均荧光强度增加.与6 Gy照射组小鼠相比,6Gy照射＋富氢水组小鼠的胸腺细胞中活性氧水平下降,早期凋亡和晚期凋亡的细胞比例降低,细胞内γ-H2AX平均荧光强度则是下降的,差异均具有统计学意义.结论 富氢水可以有效减轻电离辐射引起的胸腺细胞损伤,为富氢水作为辐射损伤防护剂提供了实验依据.
Objective The apoptosis and DNA damage of thymus cells in mice with total body irradiation of 6 Gy were investigated to determine the effect of hydrogen-rich water on reactive oxygen species （ROS）.Methods A total of 15 mice were divided into three groups, namely, un-irradiated control group, 6 Gy irradiation group, and 6 Gy ＋hydrogen-rich water group.Up to 0.5 ml of hydrogen-rich water was administered to the mice in 6 Gy＋hydrogen-rich water group 10 min before irradiation, which was provided once a day within 7 days after irradiation, and the other two groups were administered water instead.Thymus cells were collected at 4, 7, and 15 days after irradiation.Levels of ROS, apoptosis, and mean fluorescence intensity （MFI） of phosphorylated histone H2AX （γ-H2AX） were detected by flow cytometry.Results ROS levels, early （Annexin V-positive and propidium iodide （PI）-negative） and late apoptotic （Annexin V-positive and PI-positive） cells, and MFI of γ-H2AX increased in the thymus of mice irradiated with 6 Gy compared with those in the un-irradiated control group.By contrast, the levels of ROS, apoptotic cells, and MFI of γ-H2AX significantly declined in the thymus of mice administered with 6 Gy ＋ hydrogen-rich water compared with those in the irradiated mice.Conclusion Hydrogen-rich water exerted a promising protective effect on radiation-induced thymus injury.Therefore, hydrogen-rich water is a potential radioprotective agent.
出处
《国际放射医学核医学杂志》
2015年第5期358-362,共5页
International Journal of Radiation Medicine and Nuclear Medicine
基金
国家自然科学基金面上项目（81112721）
国家自然科学基金青年项目（81402633）
中国医学科学院放射医学研究所发展基金（1524,1544）
关键词
辐射
电离
活性氧
细胞凋亡
DNA损伤
富氢水
胸腺细胞
Radiation,ionizing
Reactive oxygen species
Apoptosis
DNA damage
Hydrogen-rich water
Thymus cells
