摘要
目的探讨PIAsxα对鼻咽癌细胞凋亡和成瘤能力的影响及可能机制。方法采用实时定量PCR,流式细胞术以及裸鼠成瘤实验分析PIAsxα在鼻咽癌组织中的表达情况,PIAsxα对鼻咽癌细胞系CNE1凋亡和cyclin D、CDK表达及其成瘤能力的影响。结果鼻咽癌组织中PIAsxαm RNA的表达水平显著低于邻近正常鼻咽组织。过表达PIAsxα的CNE1细胞凋亡率及G2/M期细胞比例均明显增高,cyclin D1、cyclin D3、CDK4、CDK6和CDK8的m RNA表达均显著降低且在裸鼠体内的成瘤能力亦受到抑制。结论 PIAsxα的表达下调可能通过增加cyclin D和CDK的表达,抑制鼻咽癌细胞的凋亡,促进鼻咽癌的发生和发展。
Objective To investigate the effect and possible mechanism of PIAsxαon the apoptosis and tumorigenesis of nasopharyngeal carcinoma cells. Method Real-time quantitative PCR, flow cytometry and nude mice tumor forming experiment were applied to analyze the expression of PIAsxαin nasopharyngeal carcinoma （NPC）, as well as the impacts of PIAsxαon CNE1 apoptosis, expressions of cyclin D and CDK, and tumorigenicity. Results The mRNA expression levels of PIAsxαin NPC were significantly lower than those in adjacent normal tissue of nasopharyngeal carcinoma. The apoptotic rate and the percentage of G2/M phase cells of PIAsxαoverexpressed CNE1 cells were significantly increased. The mRNA expressions of cyclin D1, cyclin D3, CDK4, CDK6 and CDK8 were significantly decreased. The tumori-genicity in nude mice of PIAsxαoverexpressed CNE1 cells were also significantly inhibited. Conclusion Down-regulation of PIAsxαcould enhance the expressions of cyclin D and CDK, inhibit the apoptosis of nasopharyngeal carcinoma cells and promote the development of na-sopharyngeal carcinoma.
出处
《肿瘤药学》
CAS
2015年第5期353-356,共4页
Anti-Tumor Pharmacy
